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RNA聚合酶在大肠杆菌半乳糖操纵子P1启动子的碱基对-49上游形成重要接触。

RNA polymerase makes important contacts upstream from base pair -49 at the Escherichia coli galactose operon P1 promoter.

作者信息

Busby S, Spassky A, Chan B

出版信息

Gene. 1987;53(2-3):145-52. doi: 10.1016/0378-1119(87)90002-3.

DOI:10.1016/0378-1119(87)90002-3
PMID:3038692
Abstract

A G

C to T:A transversion at bp position -19 in the gal operon promoter region relieves the dependence of galP1 promoter activity on the cAMP-CRP complex. Deletion analysis shows that expression from the promoter is decreased on replacement of the sequence between 49 and 54 bp upstream from the P1 start point. Moreover, protection experiments show that RNA polymerase interacts with this region in open complexes at P1. We propose that this contact is necessary for optimal P1 activity; point mutations in the gal promoter region can alter DNA flexibility and hence the strength of this contact; CRP factor activates P1 transcription by favouring formation of this contact; and the gal repressor blocks P1 activity by binding to this zone.

摘要

在半乳糖操纵子启动子区域第 -19 碱基对位置发生的 G:C 到 T:A 的颠换消除了半乳糖 P1 启动子活性对 cAMP - CRP 复合物的依赖性。缺失分析表明，在替换 P1 起始点上游 49 至 54 碱基对之间的序列时，启动子的表达会降低。此外，保护实验表明，RNA 聚合酶在 P1 的开放复合物中与该区域相互作用。我们提出，这种接触对于 P1 的最佳活性是必要的；半乳糖启动子区域的点突变可以改变 DNA 的柔韧性，从而改变这种接触的强度；CRP 因子通过促进这种接触的形成来激活 P1 转录；而半乳糖阻遏物通过结合到该区域来阻断 P1 活性。

相似文献

1
RNA polymerase makes important contacts upstream from base pair -49 at the Escherichia coli galactose operon P1 promoter.RNA聚合酶在大肠杆菌半乳糖操纵子P1启动子的碱基对-49上游形成重要接触。
Gene. 1987;53(2-3):145-52. doi: 10.1016/0378-1119(87)90002-3.
2
Location of the C-terminal domain of the RNA polymerase alpha subunit in different open complexes at the Escherichia coli galactose operon regulatory region.大肠杆菌半乳糖操纵子调控区域不同开放复合物中RNA聚合酶α亚基C末端结构域的定位
Nucleic Acids Res. 1996 Jun 15;24(12):2242-51. doi: 10.1093/nar/24.12.2243.
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Interactions between the cyclic AMP receptor protein and the alpha subunit of RNA polymerase at the Escherichia coli galactose operon P1 promoter.环腺苷酸受体蛋白与大肠杆菌半乳糖操纵子P1启动子处RNA聚合酶α亚基之间的相互作用。
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Regulation of open complex formation at the Escherichia coli galactose operon promoters. Simultaneous interaction of RNA polymerase, gal repressor and CAP/cAMP.大肠杆菌半乳糖操纵子启动子处开放复合物形成的调控。RNA聚合酶、半乳糖阻遏物和CAP/cAMP的同时相互作用。
J Mol Biol. 1992 Mar 5;224(1):15-29. doi: 10.1016/0022-2836(92)90573-3.
5
Two catabolite activator protein molecules bind to the galactose promoter region of Escherichia coli in the presence of RNA polymerase.在RNA聚合酶存在的情况下，两个分解代谢物激活蛋白分子结合到大肠杆菌的半乳糖启动子区域。
Proc Natl Acad Sci U S A. 1983 Mar;80(6):1594-8. doi: 10.1073/pnas.80.6.1594.
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Recognition of nucleotide sequences at the Escherichia coli galactose operon P1 promoter by RNA polymerase.RNA聚合酶对大肠杆菌半乳糖操纵子P1启动子核苷酸序列的识别。
Gene. 1989 Dec 14;84(2):227-36. doi: 10.1016/0378-1119(89)90496-4.
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Transcription initiation at the Escherichia coli galactose operon promoters in the absence of the normal -35 region sequences.在缺乏正常-35区域序列的情况下，大肠杆菌半乳糖操纵子启动子处的转录起始。
J Biol Chem. 1986 Dec 5;261(34):16043-8.
8
Interactions of RNA polymerase and the cyclic AMP receptor protein on DNA of the E. coli galactose operon.RNA聚合酶与环腺苷酸受体蛋白在大肠杆菌半乳糖操纵子DNA上的相互作用。
Nucleic Acids Res. 1983 Aug 11;11(15):5165-80. doi: 10.1093/nar/11.15.5165.
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Alterations in the binding site of the cyclic AMP receptor protein at the Escherichia coli galactose operon regulatory region.环腺苷酸受体蛋白在大肠杆菌半乳糖操纵子调控区域结合位点的改变。
Biochem J. 1988 Aug 1;253(3):809-18. doi: 10.1042/bj2530809.
10
Studies with the Escherichia coli galactose operon regulatory region carrying a point mutation that simultaneously inactivates the two overlapping promoters. Interactions with RNA polymerase and the cyclic AMP receptor protein.对携带一个点突变的大肠杆菌半乳糖操纵子调控区进行的研究，该点突变同时使两个重叠启动子失活。与RNA聚合酶和环磷酸腺苷受体蛋白的相互作用。
FEBS Lett. 1987 Jul 13;219(1):189-96. doi: 10.1016/0014-5793(87)81214-0.

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