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两个具有延伸 -10 序列的大肠杆菌启动子处开放复合物形成的温度依赖性

Temperature-dependence of open-complex formation at two Escherichia coli promoters with extended -10 sequences.

作者信息

Burns H D, Belyaeva T A, Busby S J, Minchin S D

机构信息

School of Biochemistry, University of Birmingham, U.K.

出版信息

Biochem J. 1996 Jul 1;317 ( Pt 1)(Pt 1):305-11. doi: 10.1042/bj3170305.

DOI:10.1042/bj3170305
PMID:8694780
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1217479/
Abstract

We have studied the formation of open complexes between purified RNA polymerase from Escherichia coli and DNA fragments carrying the galP1 promoter, a promoter with an extended -10 region. Unusually, these complexes are formed readily at low temperatures. This low-temperature opening is unaffected by deletions of either upstream or downstream promoter sequences. We conclude that low-temperature open-complex formation is due to specific base sequences in and just upstream of the extended -10 region. In contrast, open complexes are not formed at low temperatures with DNA fragments carrying the E. coli cysG promoter, which also has an extended -10 region. This demonstrates that an extended -10 sequence alone is not sufficient for low-temperature opening. Additionally, we report the temperature dependence of a hybrid galP1-cysG promoter, the related galP2 and galP3 promoters and a derivative of galP1 with an improved -10 hexamer sequence.

摘要

我们研究了来自大肠杆菌的纯化RNA聚合酶与携带galP1启动子（一个具有延伸 -10区域的启动子）的DNA片段之间开放复合物的形成。不同寻常的是，这些复合物在低温下很容易形成。这种低温开放不受上游或下游启动子序列缺失的影响。我们得出结论，低温开放复合物的形成是由于延伸 -10区域及其上游的特定碱基序列。相比之下，携带大肠杆菌cysG启动子（其也具有延伸 -10区域）的DNA片段在低温下不会形成开放复合物。这表明仅延伸 -10序列不足以实现低温开放。此外，我们报告了杂合galP1 - cysG启动子、相关的galP2和galP3启动子以及具有改进 -10六聚体序列的galP1衍生物的温度依赖性。

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本文引用的文献

1
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Biochem J. 1993 Feb 1;289 ( Pt 3)(Pt 3):771-5. doi: 10.1042/bj2890771.
2
The minus 35-recognition region of Escherichia coli sigma 70 is inessential for initiation of transcription at an "extended minus 10" promoter.大肠杆菌σ70的-35识别区域对于在“延伸的-10”启动子处起始转录并非必需。
J Mol Biol. 1993 Jul 20;232(2):406-18. doi: 10.1006/jmbi.1993.1400.
3
Coupling of local folding to site-specific binding of proteins to DNA.蛋白质局部折叠与蛋白质与DNA位点特异性结合的偶联。
Science. 1994 Feb 11;263(5148):777-84. doi: 10.1126/science.8303294.
4
Promoters responsive to DNA bending: a common theme in prokaryotic gene expression.对DNA弯曲有反应的启动子：原核生物基因表达中的一个共同主题。
Microbiol Rev. 1994 Jun;58(2):268-90. doi: 10.1128/mr.58.2.268-290.1994.
5
Interactions between the cyclic AMP receptor protein and the alpha subunit of RNA polymerase at the Escherichia coli galactose operon P1 promoter.环腺苷酸受体蛋白与大肠杆菌半乳糖操纵子P1启动子处RNA聚合酶α亚基之间的相互作用。
Nucleic Acids Res. 1994 Oct 25;22(21):4375-80. doi: 10.1093/nar/22.21.4375.
6
Thermal energy requirement for strand separation during transcription initiation: the effect of supercoiling and extended protein DNA contacts.转录起始过程中链分离所需的热能：超螺旋和扩展的蛋白质-DNA接触的影响
Nucleic Acids Res. 1994 Sep 25;22(19):3840-5. doi: 10.1093/nar/22.19.3840.
7
The Escherichia coli cysG promoter belongs to the 'extended -10' class of bacterial promoters.大肠杆菌cysG启动子属于细菌启动子的“扩展-10”类别。
Biochem J. 1993 Dec 15;296 ( Pt 3)(Pt 3):851-7. doi: 10.1042/bj2960851.
8
Open complex formation by Escherichia coli RNA polymerase: the mechanism of polymerase-induced strand separation of double helical DNA.
Mol Microbiol. 1995 Jun;16(5):817-24. doi: 10.1111/j.1365-2958.1995.tb02309.x.
9
Mapping of single-stranded regions in duplex DNA at the sequence level: single-strand-specific cytosine methylation in RNA polymerase-promoter complexes.双链DNA中单链区域在序列水平上的定位：RNA聚合酶-启动子复合物中的单链特异性胞嘧啶甲基化
Proc Natl Acad Sci U S A. 1983 May;80(9):2544-8. doi: 10.1073/pnas.80.9.2544.
10
Mechanism and control of transcription initiation in prokaryotes.原核生物转录起始的机制与调控
Annu Rev Biochem. 1985;54:171-204. doi: 10.1146/annurev.bi.54.070185.001131.