两个具有延伸 -10 序列的大肠杆菌启动子处开放复合物形成的温度依赖性
Temperature-dependence of open-complex formation at two Escherichia coli promoters with extended -10 sequences.
作者信息
Burns H D, Belyaeva T A, Busby S J, Minchin S D
机构信息
School of Biochemistry, University of Birmingham, U.K.
出版信息
Biochem J. 1996 Jul 1;317 ( Pt 1)(Pt 1):305-11. doi: 10.1042/bj3170305.
Abstract
We have studied the formation of open complexes between purified RNA polymerase from Escherichia coli and DNA fragments carrying the galP1 promoter, a promoter with an extended -10 region. Unusually, these complexes are formed readily at low temperatures. This low-temperature opening is unaffected by deletions of either upstream or downstream promoter sequences. We conclude that low-temperature open-complex formation is due to specific base sequences in and just upstream of the extended -10 region. In contrast, open complexes are not formed at low temperatures with DNA fragments carrying the E. coli cysG promoter, which also has an extended -10 region. This demonstrates that an extended -10 sequence alone is not sufficient for low-temperature opening. Additionally, we report the temperature dependence of a hybrid galP1-cysG promoter, the related galP2 and galP3 promoters and a derivative of galP1 with an improved -10 hexamer sequence.
摘要
我们研究了来自大肠杆菌的纯化RNA聚合酶与携带galP1启动子（一个具有延伸 -10区域的启动子）的DNA片段之间开放复合物的形成。不同寻常的是，这些复合物在低温下很容易形成。这种低温开放不受上游或下游启动子序列缺失的影响。我们得出结论，低温开放复合物的形成是由于延伸 -10区域及其上游的特定碱基序列。相比之下，携带大肠杆菌cysG启动子（其也具有延伸 -10区域）的DNA片段在低温下不会形成开放复合物。这表明仅延伸 -10序列不足以实现低温开放。此外，我们报告了杂合galP1 - cysG启动子、相关的galP2和galP3启动子以及具有改进 -10六聚体序列的galP1衍生物的温度依赖性。
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