Giannini A, Zampi G, Bartoloni F, Omer S
Hepatogastroenterology. 1987 Jun;34(3):95-7.
Morphometrical analysis of different types of liver cells was performed in cases of chronic active hepatitis (CAH), liver cirrhosis (LC), and hepatocellular carcinoma (HCC). The area and a form factor (form AR) of nuclei and cytoplasms were determined, and the nucleo-cytoplasmic ratio (N/C) was calculated in normal hepatocytes, HBsAg-positive cells, large dysplastic cells, cancer cells and in a liver cell population identifiable as "small dysplastic cells" (small cells). The nucleo-cytoplasmic ratio of the small cells and of the neoplastic cells was roughly the same and the highest. Large dysplastic cells showed a small nucleo-cytoplasmic ratio, almost comparable with that of normal hepatocytes and similar to that of HBsAg-positive cells. Since cellular precursors of liver cancer are expected to have a nucleo-cytoplasmic ratio similar to that of neoplastic cells, our morphometrical evaluation indicates small cells as the true precancerous cells; liver cell dysplasia (large dysplastic cells), as described by Anthony et al., should not be considered as a true preneoplastic change.
对慢性活动性肝炎（CAH）、肝硬化（LC）和肝细胞癌（HCC）病例进行了不同类型肝细胞的形态计量分析。测定了细胞核和细胞质的面积及形态因子（形态AR），并计算了正常肝细胞、HBsAg阳性细胞、大的发育异常细胞、癌细胞以及可识别为“小的发育异常细胞”（小细胞）的肝细胞群体的核质比（N/C）。小细胞和肿瘤细胞的核质比大致相同且最高。大的发育异常细胞的核质比小，几乎与正常肝细胞的核质比相当，且与HBsAg阳性细胞的核质比相似。由于预计肝癌的细胞前体具有与肿瘤细胞相似的核质比，我们的形态计量评估表明小细胞是真正的癌前细胞；Anthony等人所描述的肝细胞发育异常（大的发育异常细胞）不应被视为真正的肿瘤前变化。
