Luo Anling, Zheng Youli, Chen Xinxin, Cong Fengsong
Department of Biochemistry and Molecular Biology, School of life Sciences and Biotechnology, Shanghai Jiaotong University, Shanghai, China.
Biochem Mol Biol Educ. 2018 Nov;46(6):644-651. doi: 10.1002/bmb.21177. Epub 2018 Nov 2.
Laboratory exercises focused on protein quantification are frequently conducted in traditional undergraduate biochemistry laboratory curriculum. The laboratory course described here is designed to provide students with experience in measurement of protein content in milk powder by moving reaction boundary titration (MRBT), a new rapid technique for total protein content determination in milk. In addition, this approach is weakly influenced by nonprotein nitrogen reagents such as melamine and urea. The course was done as three weekly laboratory exercises. First, students established a standard curve for milk protein concentration by MRBT method. Then, students investigated the influence of nonprotein nitrogen reagents on MRBT method. Finally, students made a comparison among three different protein quantification methods (MRBT, Biuret, and Kjeldahl method). From the experiments, students grasped the concept and advantages of MRBT and deepened the understanding of protein quantification. This course offer students the opportunity to be exposed to an advanced technique, which may have practical significance to their future study and work in the life science field. © 2018 International Union of Biochemistry and Molecular Biology, 46(6):644-651, 2018.
专注于蛋白质定量的实验练习在传统的本科生物化学实验课程中经常开展。这里描述的实验课程旨在通过移动反应边界滴定法（MRBT）为学生提供测量奶粉中蛋白质含量的经验，MRBT是一种测定牛奶中总蛋白质含量的新型快速技术。此外，这种方法受三聚氰胺和尿素等非蛋白质氮试剂的影响较小。该课程以每周三次实验练习的形式进行。首先，学生通过MRBT方法建立牛奶蛋白质浓度的标准曲线。然后，学生研究非蛋白质氮试剂对MRBT方法的影响。最后，学生对三种不同的蛋白质定量方法（MRBT、双缩脲法和凯氏定氮法）进行比较。通过这些实验，学生掌握了MRBT的概念和优点，并加深了对蛋白质定量的理解。本课程为学生提供了接触先进技术的机会，这对他们未来在生命科学领域的学习和工作可能具有实际意义。© 2018国际生物化学与分子生物学联盟，46(6):644 - 651, 2018。
