Shelby Shameka J
Department of Chemistry, Biochemistry, and Physics, Florida Southern College, Lakeland, Florida 33801.
Biochem Mol Biol Educ. 2019 May;47(3):220-227. doi: 10.1002/bmb.21227. Epub 2019 Feb 22.
Due to resource limitations at predominantly undergraduate institutions, research opportunities for non-senior students can be limited. To provide opportunities for a variety of students to gain exposure to research, a course-based undergraduate research experience (CURE) was designed and conducted. Coupled inquiry was used to allow underclassmen and upperclassmen to participate. Students first utilized a bioinformatics approach to develop hypotheses concerning protein interactions with the receptor Mer tyrosine kinase (MERTK). Students designed experiments to identify specific sites of interactions with SH2-domain proteins utilizing an assortment of basic biochemical techniques. The semester culminated in students testing their hypotheses and producing manuscripts. Underclassmen that participated in the course also benefitted from mentor-mentee relationships developed with upperclassmen due to the collaborative nature of the course. The structure of the course also allows for further studies to be conducted based on novel findings and is highly adaptable to receptor tyrosine kinases found in other tissue types. © 2019 International Union of Biochemistry and Molecular Biology, 47(3):220-227, 2019.
由于主要是本科院校的资源有限，非高年级学生的研究机会可能会受到限制。为了为各类学生提供接触研究的机会，设计并开展了一门基于课程的本科研究体验课程（CURE）。采用合作探究的方式让低年级和高年级学生都能参与进来。学生们首先利用生物信息学方法提出有关蛋白质与受体Mer酪氨酸激酶（MERTK）相互作用的假设。学生们设计实验，运用各种基础生化技术来确定与SH2结构域蛋白相互作用的特定位点。学期结束时，学生们对自己的假设进行检验并撰写论文。由于课程的协作性质，参与该课程的低年级学生还受益于与高年级学生建立的师徒关系。该课程的结构还允许基于新发现开展进一步研究，并且高度适用于其他组织类型中发现的受体酪氨酸激酶。© 2019国际生物化学与分子生物学联盟，47(3):220 - 227, 2019。
