Graduate School of Health and Sports Science, Juntendo University, Inzai, Chiba 270-1695, Japan.
Faculty of Education, Yamaguchi University, Yamaguchi, Yamaguchi 753-8513, Japan.
J Zhejiang Univ Sci B. 2018;19(11):844-852. doi: 10.1631/jzus.B1800076.
We examined the effect of a combination of astaxanthin (AX) supplementation, repeated heat stress, and intermittent reloading (IR) on satellite cells in unloaded rat soleus muscles. Forty-nine male Wistar rats (8-week-old) were divided into control, hind-limb unweighting (HU), IR during HU, IR with AX supplementation, IR with repeated heat stress (41.0-41.5 °C for 30 min), and IR with AX supplementation and repeated heat stress groups. After the experimental period, the antigravitational soleus muscle was analyzed using an immunohistochemical technique. Our results revealed that the combination of dietary AX supplementation and heat stress resulted in protection against disuse muscle atrophy in the soleus muscle. This protective effect may be partially due to a higher satellite cell number in the atrophied soleus muscle in the IR/AX/heat stress group compared with the numbers found in the other groups. We concluded that the combination treatment with dietary AX supplementation and repeated heat stress attenuates soleus muscle atrophy, in part by increasing the number of satellite cells.
我们研究了虾青素（AX）补充、反复热应激和间歇性加载（IR）联合对去负荷大鼠比目鱼肌卫星细胞的影响。49 只雄性 Wistar 大鼠（8 周龄）分为对照组、后肢去负荷（HU）组、HU 期间 IR 组、IR 时 AX 补充组、IR 时重复热应激（41.0-41.5°C 30 分钟）组和 IR 时 AX 补充和重复热应激组。实验结束后，采用免疫组织化学技术分析抗重力比目鱼肌。结果表明，饮食 AX 补充和热应激的联合作用可防止比目鱼肌废用性萎缩。这种保护作用可能部分归因于 IR/AX/热应激组萎缩比目鱼肌中的卫星细胞数量高于其他组。我们得出结论，饮食 AX 补充和重复热应激的联合治疗可减轻比目鱼肌萎缩，部分原因是增加了卫星细胞的数量。
