Bosch M L, Fei H, Bontrop R E, Gerrets R, Tilanus M G, Termijtelen A, Giphart M J
Hum Immunol. 1987 Jun;19(2):91-103. doi: 10.1016/0198-8859(87)90097-8.
We have studied HLA-DQ encoded antigens from HLA-DRw6 homozygous cells and analyzed the DQ region at the DNA level. HLA-DQ molecules were isolated from EBV transformed B-cell lines and analyzed for DQ alpha and DQ beta polymorphism. From the same set of cells, DNA was isolated and analyzed for RFLP. Polymorphism could be detected by both techniques, i.e., on the protein level and on the DNA. The variation in pI of the DQ alpha and beta chains correlated with the polymorphism as detected by HTC typing, as did the variation in molecular weight of the bands hybridizing to DQ specific cDNA probes; identical patterns were detected for cells of one HLA-D specificity and different patterns for different HLA-D types. Additionally, DQ reactive PLT reagents were raised against DRw6 positive cells. Panel studies revealed that these DQ reactive proliferative T cells can discriminate between the polymorphic DQ antigens on cells with different HLA-D specificities.
我们研究了来自HLA - DRw6纯合细胞的HLA - DQ编码抗原，并在DNA水平分析了DQ区域。从EBV转化的B细胞系中分离出HLA - DQ分子，并分析其DQα和DQβ多态性。从同一组细胞中分离出DNA并分析其RFLP。两种技术都能检测到多态性，即在蛋白质水平和DNA水平。DQα和β链的pI变化与HTC分型检测到的多态性相关，与DQ特异性cDNA探针杂交的条带分子量变化也相关；对于一种HLA - D特异性的细胞检测到相同的模式，而对于不同的HLA - D类型则检测到不同的模式。此外，针对DRw6阳性细胞制备了DQ反应性PLT试剂。分组研究表明，这些DQ反应性增殖性T细胞能够区分具有不同HLA - D特异性细胞上的多态性DQ抗原。
