Glassman A B, Bennett C E
Ann Clin Lab Sci. 1977 Nov-Dec;7(6):519-23.
B and T lymphocytes separation by use of various cell markers had been previously described. These differential lymphocyte subpopulation counts have been helpful in elucidating immunodeficiency, lymphoproliferative and other disease states. Expanding clinical utility has moved these assays into the general laboratory for routine determination. Specific methodology for differentiation of B and T cell subpopulations is described. The mean T cell population is 68.1 percent +/- 4.2 and the mean B cell population is 8.4 percent +/- 2.1 for 30 ambulatory normals. A bi-modal distribution of T and B lymphocyte cell lines is noted in a family with a high incidence of carcinoma. Preliminary data on patients in the diabetic clinic of the Medical University of South Carolina shows a statistically significant difference in the T cell population of diabetics versus normal.
此前已有利用各种细胞标志物分离B淋巴细胞和T淋巴细胞的相关描述。这些淋巴细胞亚群的差异计数有助于阐明免疫缺陷、淋巴增殖性疾病及其他疾病状态。不断扩大的临床应用使得这些检测方法进入普通实验室进行常规测定。本文描述了区分B细胞和T细胞亚群的具体方法。30名门诊正常受试者的T细胞平均比例为68.1%±4.2%，B细胞平均比例为8.4%±2.1%。在一个癌症高发家族中，观察到T淋巴细胞系和B淋巴细胞系的双峰分布。南卡罗来纳医科大学糖尿病门诊患者的初步数据显示，糖尿病患者与正常人的T细胞比例存在统计学上的显著差异。
