Tedder T F, Crain M J, Kubagawa H, Clement L T, Cooper M D
J Immunol. 1985 Sep;135(3):1786-91.
The differentiation status of T and B cells was evaluated in patients with common variable immunodeficiency (CVI), selective IgA deficiency (IgA), X-linked agammaglobulinemia (XLA), and the acquired immune deficiency syndrome (AIDS) with the use of conventional lymphocyte markers and four new monoclonal antibodies that identify lymphocyte subpopulations. These antibodies are HB 4, which identifies a subpopulation of resting B cells; HB 5, which identifies the C3d/EBV receptor on mature B cells; HB 7, which identifies immature B lymphocytes; and HB 10, which reacts with virgin but not activated or memory T cells. T and B cells from the IgA patients typically had normal phenotypic profiles, whereas diverse patterns of lymphocyte maturation were observed in CVI. In 11 of 16 CVI patients, B cells had normal antigenic phenotypes. Although B cells from four other CVI patients had normal frequencies of HB 5 and HB 7 antigen expression, few expressed the HB 4 antigen, suggesting that they were activated. In contrast, a large percentage of B cells from one CVI patient were of an immature phenotype. The expression of the HB 10 antigen by T cells in CVI patients was also variable, being normal in 10 of 16 patients, yet significantly decreased in six others. The vast majority of the limited numbers of IgM B cells from five XLA patients (greater than 100-fold reduction) has an immature phenotype (HB 4-5-7+). Interestingly, the circulating T cells in XLA patients were phenotypically similar to those in normal newborns, suggesting that T cell immaturity or defective T cell activation may occur in these B cell-deficient individuals. Circulating B cells from AIDS patients were mostly HB 7-, with variable expression of the HB 4 antigen and significantly decreased expression of the HB 5 antigen. Most of the T cells from AIDS patients were HB 10-, and thus appeared to be activated.
利用传统淋巴细胞标志物和四种可识别淋巴细胞亚群的新型单克隆抗体，对常见变异型免疫缺陷（CVI）、选择性IgA缺陷（IgA）、X连锁无丙种球蛋白血症（XLA）以及获得性免疫缺陷综合征（AIDS）患者的T细胞和B细胞分化状态进行了评估。这些抗体分别是：HB 4，可识别静息B细胞亚群；HB 5，可识别成熟B细胞上的C3d/EBV受体；HB 7，可识别未成熟B淋巴细胞；HB 10，可与初始T细胞而非活化T细胞或记忆T细胞发生反应。IgA患者的T细胞和B细胞通常具有正常的表型特征，而在CVI患者中观察到了多种淋巴细胞成熟模式。16例CVI患者中有11例的B细胞具有正常的抗原表型。尽管另外4例CVI患者的B细胞HB 5和HB 7抗原表达频率正常，但很少表达HB 4抗原，提示它们处于活化状态。相比之下，1例CVI患者的大部分B细胞具有未成熟表型。CVI患者T细胞中HB 10抗原的表达也存在差异，16例患者中有10例正常，另外6例则显著降低。5例XLA患者数量有限的IgM B细胞绝大多数（减少超过100倍）具有未成熟表型（HB 4-5-7+）。有趣的是，XLA患者的循环T细胞在表型上与正常新生儿相似，提示在这些B细胞缺陷个体中可能发生T细胞未成熟或T细胞活化缺陷。AIDS患者的循环B细胞大多为HB 7阴性，HB 4抗原表达可变，HB 5抗原表达显著降低。AIDS患者的大多数T细胞为HB 10阴性，因此似乎处于活化状态。
