探索热带印度山羊品种对捻转血矛线虫混合自然感染的抗性/易感性的分子基础。
Exploring the molecular basis of resistance/ susceptibility to mixed natural infection of Haemonchus contortus in tropical Indian goat breed.
机构信息
Division of Animal Genetics, ICAR- Indian Veterinary Research Institute (IVRI), Izatnagar, Bareilly, U.P., India.
Division of Animal Genetics, ICAR- Indian Veterinary Research Institute (IVRI), Izatnagar, Bareilly, U.P., India.
出版信息
Vet Parasitol. 2018 Oct 15;262:6-10. doi: 10.1016/j.vetpar.2018.09.003. Epub 2018 Sep 10.
The present investigation was carried out with the objective to identify putative candidate genes / Quantitative trait loci for resistance / susceptibility towards Haemonchus infestation in tropical goat breed (Rohilkhandi goat) of India. The mean faecal egg count (FEC) and packed cell volume (PCV) of the population were 142.78 ± 22.54 epg (eggs per gram) and 31.73% ± 0.49, respectively. Grouping of animals as per dot ELISA test showed 41.33% (n = 124) positive and 58.66% (n = 176) negative for Haemonchus infestation. The microsatellite loci DYA and ODRB1.2 were significantly associated (P ≤ 0.05) to parasite resistance. The locus DYA showed significant association with log FEC and dot ELISA and the locus ODRB1.2 showed significant association with log FEC, PCV and dot ELISA at P ≤ 0.05. Real time expression profiling revealed that the susceptible group (high FEC group) had 11.1-fold more expression of IFNγ mRNA (Th cytokine) and 0.11-fold lower expression of IL-10 mRNA (Th cytokine), which was found to be statistically significant (P ≤ 0.05).
本研究旨在鉴定印度热带山羊品种（罗希兰迪山羊）对感染旋毛虫的抗性/易感性的候选基因/数量性状位点。该群体的平均粪便虫卵计数（FEC）和红细胞压积（PCV）分别为 142.78 ± 22.54 epg（每克卵）和 31.73% ± 0.49。根据 dot ELISA 测试对动物进行分组显示，41.33%（n = 124）对旋毛虫感染呈阳性，58.66%（n = 176）呈阴性。微卫星位点 DYA 和 ODRB1.2 与寄生虫抗性显著相关（P ≤ 0.05）。位点 DYA 与 log FEC 和 dot ELISA 显著相关，而位点 ODRB1.2 与 log FEC、PCV 和 dot ELISA 显著相关（P ≤ 0.05）。实时表达谱分析显示，易感组（高 FEC 组）IFNγ mRNA（Th 细胞因子）的表达增加了 11.1 倍，IL-10 mRNA（Th 细胞因子）的表达降低了 0.11 倍，这具有统计学意义（P ≤ 0.05）。
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