Division of Animal Genetics, Molecular Genetics Lab, ICAR-Indian Veterinary Research Institute (IVRI), Bareilly, UP, India.
Directorate of Animal Husbandry and Veterinary Services, Chennai, TN, India.
Anim Biotechnol. 2023 Jun;34(3):709-717. doi: 10.1080/10495398.2021.1998088. Epub 2021 Nov 9.
MHC gene is highly polymorphic and plays central molecule in immune system in recognition of pathogens and parasites. The present study examined the variability of MHC class II DRB exon 2 gene using microsatellite analysis, and its association with infestation in Salem Black goat population. Animals were naturally exposed to mixed infestation of endoparasites, predominantly . Pooled fecal coproculture and larval identification showed predominant presence of haemonchus (L3) larva. Fecal egg count (FEC) and packed cell volume (PCV) were used as indicator traits. All the three studied loci, , , and , were polymorphic having three, four, and four alleles and four, eight, and nine genotypes, respectively. The loci showed low to moderate values of polymorphic information content. The mean fecal egg count estimates were 477.12 ± 34.14 eggs per gram of feces and the mean PCV values were within the normal range; however, they showed negative correlation with FEC values. All the three microsatellite loci and the effect of genotypes on indicator traits were found to be statistically significant ( ≤ 0.05). The present study will be helpful in selection and breeding of goats for Haemonchosis resistance.
MHC 基因高度多态性，在识别病原体和寄生虫的免疫系统中发挥中心分子作用。本研究使用微卫星分析检测了 MHC 类 II DRB 外显子 2 基因的变异性，并研究了其与 Salem Black 山羊群体寄生虫感染的关联。动物自然暴露于混合寄生虫感染中，主要是线虫。混合粪便培养和幼虫鉴定显示，以感染副蛔虫（L3 幼虫）为主。粪便卵计数（FEC）和红细胞压积（PCV）用作指示特征。所有研究的三个位点 、 、 和 均表现出多态性，分别具有三个、四个和四个等位基因以及四个、八个和九个基因型。这些位点表现出低到中度的多态信息含量值。平均粪便卵计数估计值为每克粪便 477.12 ± 34.14 个卵，平均 PCV 值在正常范围内；然而，它们与 FEC 值呈负相关。所有三个微卫星位点和基因型对指示特征的影响均具有统计学意义（≤0.05）。本研究将有助于对副蛔虫病抗性进行山羊的选择和繁殖。
