Adams S L, Pacifici M, Boettiger D, Pallante K M
J Cell Biol. 1987 Jul;105(1):483-8. doi: 10.1083/jcb.105.1.483.
Chicken vertebral chondrocytes, which normally grow in suspension, synthesize large amounts of cartilage extracellular matrix proteins, but little fibronectin. We have analyzed the effects of both substrate attachment and transformation with a temperature-sensitive mutant of Rous sarcoma virus on fibronectin gene expression in these cells. Our experiments show that viral transformation increases fibronectin synthesis to a greater extent than substrate attachment. Furthermore, transformed chondrocytes have lost the ability to decrease fibronectin synthesis in response to suspension culture, suggesting that transformation alters the normal attachment-responsive control of fibronectin gene expression. Finally, infected substrate-attached chondrocytes shifted to the nonpermissive temperature for transformation use fibronectin RNA more efficiently in protein synthesis than cells grown under the other conditions, suggesting for the first time a role for translational control of fibronectin gene expression.
鸡椎体软骨细胞通常悬浮生长，能合成大量软骨细胞外基质蛋白，但纤连蛋白合成量很少。我们分析了底物附着以及用劳氏肉瘤病毒的温度敏感突变体进行转化对这些细胞中纤连蛋白基因表达的影响。我们的实验表明，病毒转化比底物附着更能显著增加纤连蛋白的合成。此外，转化后的软骨细胞丧失了在悬浮培养时减少纤连蛋白合成的能力，这表明转化改变了纤连蛋白基因表达正常的附着反应性调控。最后，感染后附着于底物的软骨细胞转移到不利于转化的温度条件下，与在其他条件下生长的细胞相比，其在蛋白质合成中更有效地利用纤连蛋白RNA，这首次表明了纤连蛋白基因表达的翻译调控作用。
