Perez S, Courtis N, Kokkinopoulos D, Papamichail M, Tsiapalis C M, Trangas T
J Immunol Methods. 1987 Jul 16;101(1):73-8. doi: 10.1016/0022-1759(87)90218-3.
A rapid, simple, quantitative and sensitive assay for the determination of 5'-nucleotidase has been developed. The method can be applied to both soluble and membrane bound forms of the leukocyte enzyme. Enzyme activity is determined by colorimetric estimation of NH3 released from adenosine, the product of 5'-nucleotidase activity in the presence of adenosine deaminase. The assay may be performed in microtitre plates and read with an automatic multiscan spectrophotometer. Thus it can be applied to a large number of samples for routine medical and research purposes.
已开发出一种快速、简单、定量且灵敏的测定5'-核苷酸酶的方法。该方法可应用于白细胞酶的可溶性和膜结合形式。酶活性通过比色法估算腺苷脱氨酶存在下5'-核苷酸酶活性产物腺苷释放的NH3来测定。该测定可在微量滴定板中进行，并用自动多扫描分光光度计读取结果。因此，它可应用于大量样本，用于常规医学和研究目的。
