连续流动分析法自动测定血清中5'-核苷酸酶的方法

Automated method for the determination of 5'-nucleotidase in serum by continuous flow analysis.

作者信息

van Helden W C, van der Slik W, Persijn J P, Souverijn J H

出版信息

J Clin Chem Clin Biochem. 1980 Jun;18(6):333-7. doi: 10.1515/cclm.1980.18.6.333.

DOI:10.1515/cclm.1980.18.6.333

Abstract

The manual method of Persijn & Van der Slik (J. Clin. Chem. Clin. Biochem. 6, 441-446 (1968): 7, 493-497 (1969); 8, 398-402 (1970) for the determination of serum 5'-nucleotidase has been adapted to the Auto-Analyzer II System. In the Auto-Analyzer II, the incubation temperature for the enzyme reactions is 37 degrees C, and the effective sample speed is 30/h, at a sample/wash ratio of 2:1. There is good agreement and correlation between the manual method and the Auto-Analyzer II method (equation of regression line: y = x + 0.6, correlation coefficient = 0.988; the normal range is 2.9-10.5 U/l for both methods). In routine use, within-run and between-day reproducibility are considerably smaller for the Auto-Analyzer II than for the manual method, especially when large numbers of samples are assayed.

摘要

Persijn和Van der Slik（《临床化学与临床生物化学杂志》6, 441 - 446 (1968); 7, 493 - 497 (1969); 8, 398 - 402 (1970)）测定血清5'-核苷酸酶的手工方法已被适配于自动分析仪II系统。在自动分析仪II中，酶反应的孵育温度为37摄氏度，有效样本速度为每小时30个，样本/冲洗比为2:1。手工方法与自动分析仪II方法之间具有良好的一致性和相关性（回归线方程：y = x + 0.6，相关系数 = 0.988；两种方法的正常范围均为2.9 - 10.5 U/l）。在常规使用中，自动分析仪II的批内和日间重复性比手工方法小得多，尤其是在检测大量样本时。