Lindholm-Perry Amanda K, Kuehn Larry A, McDaneld Tara G, Miles Jeremy R, Workman Aspen M, Chitko-McKown Carol G, Keele John W
USDA, ARS, U.S. Meat Animal Research Center, P.O. Box 166, Clay Center, NE, 68933, USA.
BMC Res Notes. 2018 Nov 3;11(1):786. doi: 10.1186/s13104-018-3900-x.
The purpose of this study was to evaluate potential relationships between cytokine gene expression, complete blood counts (CBC) and animals that were sick or would become sick. The CBC and the transcript abundance of cytokines and their receptors expressed in leukocytes were measured from calves at two early timepoints, and again after diagnosis with bovine respiratory disease (BRD).
Blood was collected from calves at pre-conditioning (n = 796) and weaning (n = 791) for CBC. Blood counts were also measured for the calves with BRD (n = 13), and asymptomatic calves (n = 75) after weaning. The CBC were compared for these animals at 3 time points. At diagnosis, neutrophils were higher and basophils lower in sick animals (P < 0.05). To further characterize BRD responses, transcript abundance of 84 cytokine genes were evaluated in 5 calves with BRD and 9 asymptomatic animals at all time points. There was more data for CBC than transcript abundance; hence, animal and temporary environmental correlations between CBC and transcript abundance were exploited to improve the power of the transcript abundance data. Expression of CCL16, CXCR1, CCR1 was increased in BRD positive animals compared to controls (P-corrected < 0.1). Cytokine expression data may help to provide insight into an animal's health.
本研究旨在评估细胞因子基因表达、全血细胞计数（CBC）与患病或即将患病动物之间的潜在关系。在两个早期时间点从小牛采集CBC以及白细胞中表达的细胞因子及其受体的转录本丰度，在诊断为牛呼吸道疾病（BRD）后再次采集。
在预处理时（n = 796）和断奶时（n = 791）从小牛采集血液用于CBC。还对断奶后患有BRD的小牛（n = 13）和无症状小牛（n = 75）进行了血细胞计数。在3个时间点对这些动物的CBC进行了比较。在诊断时，患病动物的中性粒细胞较高而嗜碱性粒细胞较低（P < 0.05）。为了进一步表征BRD反应，在所有时间点对5头患有BRD的小牛和9头无症状动物评估了84个细胞因子基因的转录本丰度。CBC的数据比转录本丰度的数据更多；因此，利用CBC与转录本丰度之间的动物和临时环境相关性来提高转录本丰度数据的效力。与对照组相比，BRD阳性动物中CCL16、CXCR1、CCR1的表达增加（经校正的P < 0.1）。细胞因子表达数据可能有助于深入了解动物的健康状况。
