Das I, de Belleroche J, Hirsch S
Life Sci. 1987 Aug 24;41(8):1037-41. doi: 10.1016/0024-3205(87)90693-x.
The effect of the polyamine, spermidine, on formyl-methionyl-leucyl-phenylalanine stimulated hydrolysis of polyphosphoinositides was examined in purified human neutrophils by measurement of inositol phosphate production from radioactively labelled inositol. Spermidine inhibited formyl-methionyl-leucyl-phenylalanine stimulated inositol phosphate production by neutrophil in a dose dependent manner. Inhibition of formyl-methionyl-leucyl-phenylalanine stimulated inositol phosphate accumulation by spermidine was maximal at 10 microM and the IC50 value for this effect was 4.2 microM spermidine. This action of spermidine, thought to be mediated by a membrane component other than phospholipase C, may reflect a control mechanism modulating the response of the polyphosphoinositide system.
通过测量放射性标记肌醇产生的肌醇磷酸，在纯化的人中性粒细胞中检测了多胺亚精胺对甲酰甲硫氨酰亮氨酰苯丙氨酸刺激的多磷酸肌醇水解的影响。亚精胺以剂量依赖的方式抑制中性粒细胞中甲酰甲硫氨酰亮氨酰苯丙氨酸刺激的肌醇磷酸产生。亚精胺对甲酰甲硫氨酰亮氨酰苯丙氨酸刺激的肌醇磷酸积累的抑制作用在10微摩尔时最大，该作用的半数抑制浓度（IC50）值为4.2微摩尔亚精胺。亚精胺的这种作用被认为是由磷脂酶C以外的膜成分介导的，可能反映了一种调节多磷酸肌醇系统反应的控制机制。
