Dillon S B, Murray J J, Snyderman R
Biochem Biophys Res Commun. 1987 Apr 14;144(1):264-70. doi: 10.1016/s0006-291x(87)80505-3.
Analysis of inositol phosphate formation in chemoattractant-stimulated human polymorphonuclear leukocytes demonstrated the production of inositol 1,4,5-trisphosphate, inositol 1,3,4-trisphosphate, inositol 1,3,4,5-tetrakisphosphate, inositol 1,4-bisphosphate and another inositol bisphosphate isomer not detected in unstimulated cells. Studies in cell sonicates provided evidence that the previously unidentified inositol bisphosphate isomer is produced via the degradation of inositol 1,3,4-trisphosphate. This unidentified inositol bisphosphate peak was purified by high pressure liquid chromatography, and base hydrolyzed to form a mixture of inositol monophosphate isomers. Based on these studies, the unidentified peak was identified as inositol 3,4-bisphosphate. Identification of this isomer defines a new metabolic product derived from the initial inositol 1,4,5-trisphosphate formation, and also suggests another substrate for the inositol 1-phosphatase.
对趋化因子刺激的人多形核白细胞中肌醇磷酸形成的分析表明，产生了1,4,5-三磷酸肌醇、1,3,4-三磷酸肌醇、1,3,4,5-四磷酸肌醇、1,4-二磷酸肌醇以及另一种在未刺激细胞中未检测到的肌醇二磷酸异构体。对细胞超声裂解物的研究提供了证据，表明先前未鉴定的肌醇二磷酸异构体是通过1,3,4-三磷酸肌醇的降解产生的。通过高压液相色谱法纯化了这个未鉴定的肌醇二磷酸峰，并进行碱水解以形成肌醇单磷酸异构体的混合物。基于这些研究，该未鉴定的峰被鉴定为3,4-二磷酸肌醇。这种异构体的鉴定定义了一种源自最初形成的1,4,5-三磷酸肌醇的新代谢产物，也提示了1-磷酸肌醇酶的另一种底物。
