Davies P, Smith B T, Maddalo F B, Langleben D, Tobias D, Fujiwara K, Reid L
Microvasc Res. 1987 May;33(3):300-14. doi: 10.1016/0026-2862(87)90025-2.
Pericytes and endothelial cells from the same sample of adult rat lung have been separately established in culture by use of selective growth media. The endothelial cells are positive and the pericytes negative for angiotensin-converting enzyme activity and tissue plasminogen activator. Morphologically in culture, the pericytes are similar to pericytes from bovine retina and other sites and show positive immunofluorescence to both human platelet (non-muscle) myosin and smooth muscle myosin. In this respect they resemble smooth muscle cells grown from the rat main pulmonary artery, but lack the myofilaments and dense bodies characteristic of muscle cells. Lung endothelial cells and fibroblasts are positive only for platelet myosin. Pericytes in culture demonstrate an unusual growth response to endothelial substrate, obtained by removing confluent endothelial monolayers with nonionic detergent or alkali. When plated onto this material at low density, pericyte growth is inhibited. By contrast, the substrate stimulates the growth of endothelial cells and has no effect on smooth muscle cells. Initial attachment of endothelial cells and pericytes to the substrate is similar.
通过使用选择性生长培养基，已分别在培养物中建立了来自成年大鼠肺同一样本的周细胞和内皮细胞。内皮细胞对血管紧张素转换酶活性和组织纤溶酶原激活物呈阳性，而周细胞呈阴性。在培养中，周细胞在形态上与来自牛视网膜和其他部位的周细胞相似，并且对人血小板（非肌肉）肌球蛋白和平滑肌肌球蛋白均显示阳性免疫荧光。在这方面，它们类似于从大鼠主肺动脉生长的平滑肌细胞，但缺乏肌肉细胞特有的肌丝和致密体。肺内皮细胞和成纤维细胞仅对血小板肌球蛋白呈阳性。培养中的周细胞对通过用非离子洗涤剂或碱去除汇合的内皮单层而获得的内皮底物表现出异常的生长反应。当以低密度接种到这种材料上时，周细胞生长受到抑制。相比之下，该底物刺激内皮细胞的生长，对平滑肌细胞没有影响。内皮细胞和周细胞对底物的初始附着是相似的。
