Cole O F, Fan T P, Lewis G P
Cell Biol Int Rep. 1986 Jun;10(6):399-405. doi: 10.1016/0309-1651(86)90034-2.
Rat aortic endothelial cells have been isolated by the explantation technique and grown in culture. They have been identified morphologically using standard staining techniques, biochemically by identification of angiotensin convertase and have been positively stained for Factor VIII-related antigen by immunofluorescence using both anti-human and anti-rat Factor VIII antibodies. The explantation technique is a successful alternative to enzyme digestion which is not applicable to rat aortic endothelial cells because of the nature of their attachment to the subendothelial layer.
大鼠主动脉内皮细胞已通过组织块培养技术分离并在培养中生长。它们已通过标准染色技术进行形态学鉴定，通过鉴定血管紧张素转换酶进行生化鉴定，并使用抗人及抗大鼠因子 VIII 抗体通过免疫荧光法对因子 VIII 相关抗原进行阳性染色。组织块培养技术是酶消化法的一种成功替代方法，由于大鼠主动脉内皮细胞与内皮下层附着的性质，酶消化法不适用于大鼠主动脉内皮细胞。
