Department of Orthopaedic Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Science, Beijing 100730, China.
Department of Orthopaedic Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Science, Beijing 100730, China.
Gene. 2019 Feb 15;685:55-61. doi: 10.1016/j.gene.2018.10.076. Epub 2018 Oct 27.
Long non-coding RNAs (lncRNAs) are recently reported to regulate the homeostasis of cartilage in osteoarthritis (OA), but their regulatory roles in OA synovium remain elusive. This study aimed to identify the differentially expressed mRNAs and lncRNAs in OA synovium and further explore the function of differentially expressed lncRNAs in OA progression through bioinformatics analysis. We started with RNA-sequencing in 5 OA synovium samples and 5 healthy controls to compare the expression of mRNAs and lncRNAs. GO analysis and KEGG pathway analysis were performed to annotate the function of differentially expressed mRNAs. Then real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was admitted in 17 osteoarthritic synovium and 18 healthy controls to confirm the changes of the expression of the selected lncRNAs. LncRNA-mRNA co-expression network was constructed to predict the potential molecular regulatory mechanisms of specifically expressed lncRNAs in OA synovium. 384 mRNAs and 17 lncRNAs were detected to be differentially expressed in OA synovium. The expressions of 4 lncRNAs were confirmed by qRT-PCR. We found the differentially expression lncRNAs could potentially regulate OA progression through pathways regarding to immune response through the lncRNA-mRNA network and further annotation for co-expression mRNAs. Our results indicated that lncRNAs may play key roles in OA synovitis and may have potential value in OA diagnosis.
长链非编码 RNA（lncRNAs）最近被报道可调节骨关节炎（OA）软骨的内稳态，但它们在 OA 滑膜中的调节作用仍不清楚。本研究旨在鉴定 OA 滑膜中差异表达的 mRNAs 和 lncRNAs，并通过生物信息学分析进一步探讨差异表达 lncRNAs 在 OA 进展中的功能。我们首先对 5 例 OA 滑膜样本和 5 例健康对照者进行 RNA-seq，比较 mRNAs 和 lncRNAs 的表达。进行 GO 分析和 KEGG 通路分析以注释差异表达 mRNAs 的功能。然后，在 17 例骨关节炎滑膜和 18 例健康对照者中进行实时定量逆转录聚合酶链反应（qRT-PCR）以确认所选 lncRNAs 的表达变化。构建 lncRNA-mRNA 共表达网络，以预测 OA 滑膜中特异性表达 lncRNAs 的潜在分子调控机制。在 OA 滑膜中检测到 384 个 mRNAs 和 17 个 lncRNAs 表达差异。通过 qRT-PCR 验证了 4 个 lncRNAs 的表达。我们发现差异表达的 lncRNAs 通过 lncRNA-mRNA 网络及其共表达 mRNAs 的进一步注释，可能通过与免疫反应相关的途径调节 OA 的进展。我们的结果表明，lncRNAs 可能在 OA 滑膜炎中发挥关键作用，并可能在 OA 诊断中具有潜在价值。
