二丁酰环磷腺苷处理的HL-60细胞中c-myc和转铁蛋白受体的转录失活

Trepel J B, Colamonici O R, Kelly K, Schwab G, Watt R A, Sausville E A, Jaffe E S, Neckers L M

Mol Cell Biol. 1987 Jul;7(7):2644-8. doi: 10.1128/mcb.7.7.2644-2648.1987.

Treatment of HL-60 cells with dibutyryl cyclic AMP induced rapid transcriptional inactivation of c-myc and the transferrin receptor. Transcriptional inactivation was followed by loss of c-myc and transferrin receptor mRNA and protein. Treated cells completed one round of proliferation, followed by growth arrest, G1 synchronization, and monocytic differentiation. These data suggest that cyclic AMP-mediated control of growth and differentiation may be achieved, at least in part, by transcriptional regulation of certain growth-associated proto-oncogenes and growth factor receptor genes.

用二丁酰环磷腺苷处理HL-60细胞可诱导c-myc和转铁蛋白受体的快速转录失活。转录失活之后是c-myc和转铁蛋白受体mRNA及蛋白质的丢失。经处理的细胞完成一轮增殖，随后生长停滞、G1期同步化并单核细胞分化。这些数据表明，环磷腺苷介导的生长和分化调控至少部分可通过某些生长相关原癌基因和生长因子受体基因的转录调控来实现。