Strome C P, Tubergen T A, Leef J L, Beaudoin R L
Bull World Health Organ. 1977;55(2-3):305-8.
A study spanning 10 years indicates that the erythrocytic stages of malarial parasites can be stored indefinitely in the vapour phase of liquid nitrogen without significant loss of infectivity. The results also suggest that loss due to the recovery procedures exceeds any loss that may have occurred during freezing and thawing. When feasible, large pools of material should be divided into smaller aliquots before storing the organisms at a low temperature, since freezing a second time produces significant losses of infectivity. Sporozoites and exoerythrocytic stages may be treated similarly, but the optimum conditions for freezing these forms have yet to be determined.
一项为期10年的研究表明，疟原虫的红细胞阶段可以无限期地保存在液氮的气相中，而不会显著丧失感染力。研究结果还表明，复苏过程造成的损失超过了冷冻和解冻过程中可能发生的任何损失。在可行的情况下，在将生物体低温保存之前，应将大量材料分成较小的等分试样，因为再次冷冻会导致感染力显著丧失。子孢子和红细胞外期可能也可采用类似方法处理，但冷冻这些形态的最佳条件尚未确定。
