Zijlstra F J, van den Broek A M, Vincent J E, Diderich P P, op de Hoek-Fes A M, Claeys M
Prostaglandins Leukot Med. 1987 May;27(2-3):151-60. doi: 10.1016/0262-1746(87)90067-9.
Macrophages, isolated from dialysis fluid of three patients with continuous ambulatory peritoneal dialysis (CAPD) at different times during peritonitis were labelled with 14C-arachidonic acid and stimulated with the calcium ionophore A23187. The main metabolites formed by 5-lipoxygenase activity were leukotriene B4 (LTB4) and 5-hydroxy-6, 9, 11, 14-eicosatetraenoic acid (5-HETE). Smaller amounts of cyclooxygenase metabolites were present and also a major compound with an elution time between 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha) and thromboxane B2 (TxB2). This substance was isolated, analyzed by GC-MS and identified as 20-hydroxy-leukotriene B4 (20-OH-LTB4). This indicates that human peritoneal macrophages obtained from CAPD not only produce leukotrienes and prostaglandins, but also the omega-hydroxylase product of LTB4, which has been demonstrated to be present in polymorphonuclear leucocytes. The activity of this enzyme was not correlated with the severity of the peritonitis.
从三名持续性非卧床腹膜透析（CAPD）患者腹膜炎不同时期的透析液中分离出巨噬细胞，用14C - 花生四烯酸标记并用钙离子载体A23187刺激。5 - 脂氧合酶活性形成的主要代谢产物是白三烯B4（LTB4）和5 - 羟基 - 6,9,11,14 - 二十碳四烯酸（5 - HETE）。存在较少量的环氧化酶代谢产物以及一种洗脱时间介于6 - 酮 - 前列腺素F1α（6 - 酮 - PGF1α）和血栓素B2（TxB2）之间的主要化合物。该物质被分离出来，通过气相色谱 - 质谱联用（GC - MS）分析并鉴定为20 - 羟基 - 白三烯B4（20 - OH - LTB4）。这表明从CAPD患者获得的人腹膜巨噬细胞不仅产生白三烯和前列腺素，还产生LTB4的ω - 羟化酶产物，该产物已被证明存在于多形核白细胞中。这种酶的活性与腹膜炎的严重程度无关。
