阳离子花菁生色团组装的上转换纳米粒子用于活细胞和斑马鱼中 HS 的检测和成像。
Cationic cyanine chromophore-assembled upconversion nanoparticles for sensing and imaging HS in living cells and zebrafish.
机构信息
Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China.
Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China.
出版信息
Biosens Bioelectron. 2019 Feb 1;126:96-101. doi: 10.1016/j.bios.2018.10.056. Epub 2018 Oct 27.
Elevated hydrogen sulfide (HS) level is closely associated with various diseases. So the sensing of HS is noteworthy for divulging its role in diagnosing these diseases. Herein, we proposed poly(acrylic acid)-modified upconversion nanoparticles assembled with cationic near-infrared cyanine chromophores (Cy7-Cl) as the nanoprobe (Cy7-UCNPs) for monitoring HS based on thiolation reactions. The presence of HS resulted into about five-fold enhancement in the luminescence intensity of Cy7-UCNPs and the nanoprobe showed a good linearity (R =0.9952) over the range of 1.0 - 90 μM. Furthermore, Cy7-UCNPs were successfully employed in sensing and imaging of exogenous and endogenous HS in live cells and zebrafish. The system shows great potential in the field of nanobiomedicine because of the many excellent properties including high sensitivity, good selectivity, and low cytotoxicity.
硫化氢 (HS) 水平升高与各种疾病密切相关。因此，HS 的检测对于揭示其在这些疾病诊断中的作用非常重要。在此，我们提出了一种基于巯基反应的纳米探针（Cy7-UCNPs），该探针由聚（丙烯酸）修饰的上转换纳米粒子与阳离子近红外菁染料（Cy7-Cl）组装而成。HS 的存在导致 Cy7-UCNPs 的荧光强度增强约五倍，并且纳米探针在 1.0-90 μM 的范围内显示出良好的线性关系（R = 0.9952）。此外，Cy7-UCNPs 成功地用于活细胞和斑马鱼中外源和内源性 HS 的传感和成像。该系统具有高灵敏度、良好的选择性和低细胞毒性等许多优异性能，因此在纳米生物医学领域具有很大的应用潜力。
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