Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice, 5, 625 00, Brno, Czech Republic.
Mikrochim Acta. 2024 Aug 21;191(9):551. doi: 10.1007/s00604-024-06615-7.
Photon-upconversion nanoparticles (UCNP) have already been established as labels for affinity assays in analog and digital formats. Here, advanced, or smart, systems based on UCNPs coated with active shells, fluorescent dyes, and metal and semiconductor nanoparticles participating in energy transfer reactions are reviewed. In addition, switching elements can be embedded in such assemblies and provide temporal and spatial control of action, which is important for intracellular imaging and monitoring activities. Demonstration and critical comments on representative approaches demonstrating the progress in the use of such UCNPs in bioanalytical assays, imaging, and monitoring of target molecules in cells are reported, including particular examples in the field of cancer theranostics.
上转换纳米粒子（UCNP）已被确立为模拟和数字格式中亲和分析的标签。在此，综述了基于涂有活性壳、荧光染料以及参与能量转移反应的金属和半导体纳米粒子的 UCNP 的先进或智能系统。此外，还可以在这些组装体中嵌入开关元件，从而实现对作用的时间和空间控制，这对于细胞内成像和监测活动非常重要。本文报道了在生物分析测定、成像以及细胞中靶分子监测中使用此类 UCNP 的代表性方法的演示和关键评论，包括癌症治疗学领域的特定实例。
