Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea.
Department of Biological Sciences, Chungnam National University, Daejeon, Korea.
Mol Cells. 2018 Nov 30;41(11):979-992. doi: 10.14348/molcells.2018.0312. Epub 2018 Nov 1.
Potato ( L.) is the third most important food crop, and breeding drought-tolerant varieties is vital research goal. However, detailed molecular mechanisms in response to drought stress in potatoes are not well known. In this study, we developed EMS-mutagenized potatoes that showed significant tolerance to drought stress compared to the wild-type (WT) 'Desiree' cultivar. In addition, changes to transcripts as a result of drought stress in WT and drought-tolerant () plants were investigated by assembly using the Illumina platform. One-week-old WT and plants were treated with -1.8 Mpa polyethylene glycol-8000, and total RNA was prepared from plants harvested at 0, 6, 12, 24, and 48 h for subsequent RNA sequencing. In total, 61,100 transcripts and 5,118 differentially expressed genes (DEGs) displaying up- or down-regulation were identified in pairwise comparisons of WT and plants following drought conditions. Transcriptome profiling showed the number of DEGs with up-regulation and down-regulation at 909, 977, 1181, 1225 and 826 between WT and plants at 0, 6, 12, 24, and 48 h, respectively. Results of KEGG enrichment showed that the drought tolerance mechanism of the plant can mainly be explained by two aspects, the 'photosynthetic-antenna protein' and 'protein processing of the endoplasmic reticulum'. We also divided eight expression patterns in four pairwise comparisons of plants ( vs ) under PEG treatment. Our comprehensive transcriptome data will further enhance our understanding of the mechanisms regulating drought tolerance in tetraploid potato cultivars.
马铃薯（L.）是世界第三大重要粮食作物，培育耐旱品种是至关重要的研究目标。然而，人们对马铃薯响应干旱胁迫的详细分子机制还知之甚少。在本研究中，我们培育了 EMS 诱变的马铃薯，与野生型（WT）‘Desiree’品种相比，其对干旱胁迫具有显著的耐受性。此外，还通过 Illumina 平台的组装，研究了 WT 和耐旱（）植株在干旱胁迫下转录本的变化。将 1 周龄的 WT 和 植株用-1.8 Mpa 聚乙二醇-8000 处理，从 0、6、12、24 和 48 h 收获的植物中提取总 RNA，用于随后的 RNA 测序。在 WT 和 植株的干旱胁迫下的两两比较中，共鉴定出 61100 个转录本和 5118 个差异表达基因（DEGs），显示上调或下调。转录组谱分析显示，在 WT 和 植株之间，在 0、6、12、24 和 48 h 时，上调和下调的 DEGs 数量分别为 909、977、1181、1225 和 826。KEGG 富集结果表明，该植物的耐旱机制主要可以用两个方面来解释，即“光合天线蛋白”和“内质网蛋白质加工”。我们还在 PEG 处理下的四个两两比较中，将 8 个表达模式划分为 4 个表达模式。我们综合的转录组数据将进一步增强我们对四倍体马铃薯品种调节耐旱性的机制的理解。
