Miller L H, Sakai R K, Romans P, Gwadz R W, Kantoff P, Coon H G
Science. 1987 Aug 14;237(4816):779-81. doi: 10.1126/science.3039658.
Foreign DNA was successfully introduced into the germline of the African mosquito vector of malaria Anopheles gambiae. Stable integration of genes into the germlines of insects had been achieved previously only in Drosophila melanogaster and related species and required the use of the P element transposon. In these experiments with Anopheles gambiae, the plasmid pUChsneo was used, which contains the selectable marker neo gene flanked by P element inverted repeats. Mosquitoes injected with this plasmid were screened for resistance to the neomycin analog G-418. A single event of plasmid insertion was recovered. Integration appears to be stable and, thus far, resistance to G-418 has been expressed for eight generations. The transformation event appears to be independent of P.
外源DNA已成功导入疟疾非洲蚊媒冈比亚按蚊的种系中。此前，基因在昆虫种系中的稳定整合仅在黑腹果蝇及相关物种中实现，且需要使用P因子转座子。在这些针对冈比亚按蚊的实验中，使用了质粒pUChsneo，它含有两侧带有P因子反向重复序列的选择标记新霉素基因。对注射了该质粒的蚊子进行了对新霉素类似物G - 418抗性的筛选。获得了一次质粒插入事件。整合似乎是稳定的，到目前为止，对G - 418的抗性已表达了八代。该转化事件似乎与P因子无关。
