Reddy Veerakishore K, Kommineni Naveen K, Padakandla Prathyusha, Togaru Harshini, Indupalli John P, Nanga Swapna P
Reader, Department of Pedodontics, CKS Teja Institute of Dental Sciences & Research, Tirupati, Andhra Pradesh, India.
Head, Department of Pedodontics, CKS Teja Institute of Dental Sciences & Research, Tirupati, Andhra Pradesh, India.
Int J Clin Pediatr Dent. 2018 Jul-Aug;11(4):288-293. doi: 10.5005/jp-journals-10005-1528. Epub 2018 Aug 1.
The goal of the study was to detect the presence of macrophage inflammatory protein (MIP)-1α and MIP-1β and to estimate their levels in gingival crevicular fluid (GCF) of children with Down syndrome.
MIP-1α and MIP-1β levels were estimated in GCF samples of 20 healthy and 20 Down syndrome individuals. Gingival status was assessed by measuring the gingival index (GI), plaque index (PI), clinical attachment level (CAL), and probing pocket depth (PPD).The GCF samples were obtained from the subjects and MIP-1α and MIP-1β levels were quantified by enzyme-linked immunosorbent assay (ELISA).
The mean MIP-1α concentrations in healthy and Down syndrome individuals were 209 and 1411 pg/μL respectively, and MIP-1α levels were 342 and 1404 pg/μL respectively.The levels of MIP-1α and MIP-1β in the GCF of subjects with Down syndrome were significantly higher than in the healthy individual, and statistically significant differences were present among the two groups.
The GCF showed dynamic changes according to the severity of periodontal disease, and the levels of MIP-1α and MIP-1β had a strong relationship with clinical parameters. The MIP-1α and MIP-1β can therefore be considered as novel biomarkers in the biological mechanism underlying the patho-genesis of periodontal disease. Reddy VK, Kommineni NK, Padakandla P, Togaru H, Indupalli JP, Nanga SP. Evaluation of Chemokines in the Gingival Crevicular Fluid of Children with Down Syndrome. Int J Clin Pediatr Dent 2018;11(4):288-293.
本研究旨在检测巨噬细胞炎性蛋白（MIP）-1α和MIP-1β的存在，并评估其在唐氏综合征患儿龈沟液（GCF）中的水平。
对20名健康个体和20名唐氏综合征个体的GCF样本进行MIP-1α和MIP-1β水平评估。通过测量牙龈指数（GI）、菌斑指数（PI）、临床附着水平（CAL）和探诊深度（PPD）来评估牙龈状况。从受试者获取GCF样本，并通过酶联免疫吸附测定（ELISA）对MIP-1α和MIP-1β水平进行定量。
健康个体和唐氏综合征个体中MIP-1α的平均浓度分别为209和1411 pg/μL，MIP-1β水平分别为342和1404 pg/μL。唐氏综合征受试者GCF中MIP-1α和MIP-1β的水平显著高于健康个体，两组之间存在统计学显著差异。
GCF根据牙周疾病的严重程度呈现动态变化，MIP-1α和MIP-1β的水平与临床参数密切相关。因此，MIP-1α和MIP-1β可被视为牙周疾病发病机制生物学机制中的新型生物标志物。雷迪VK、科米内尼NK、帕达坎德拉P、托加鲁H、因杜帕利JP、南加SP。唐氏综合征患儿龈沟液中趋化因子的评估。《国际临床儿科牙科学杂志》2018年；11（4）：288 - 293。
