Kumar Ravi Ranjan, Jadeja Vasantba J
Department of Biotechnology, Shree M. & N. Virani Science College, Kalawad Road, Rajkot 360005, Gujarat, India.
Department of Microbiology, Shree M. & N. Virani Science College, Kalawad Road, Rajkot 360005, Gujarat, India.
Bioimpacts. 2018;8(4):253-261. doi: 10.15171/bi.2018.28. Epub 2018 May 1.
: The inevitable rise of antibiotic-resistant bacteria is a global health problem. These pathogens erode the utility of available antibiotics. is one of the major causes of community-acquired infections. The aim of work was to evaluate the marine actinomycetes for production of the antibacterial agent against pathogens. : Halophilic actinomycetes were isolated, characterized and screened for production of antibacterial agent against pathogenic bacteria. The antibacterial compounds were extracted by solvent extraction and separated by TLC based bioautography. Antibacterial compound was further purified by flash chromatography followed by high-performance liquid chromatography (HPLC) techniques. The active fraction was characterized by spectroscopy techniques. The minimum inhibitory concentration of antibiotic was determined against pathogens. : A new halophilic actinomycetes strain rsk4 was isolated from marine water. It was designated as sp. based on the physiological, biochemical and 16S rDNA sequencebased characters. It was able to produce broad-spectrum antibacterial compound and exhibited significant inhibitory activities against antibiotic-resistant . The antibacterial compound was secreted optimally at 5% NaCl and neutral pH in the starch casein medium during stationary phase. The crude ethyl acetate extract was separated by chloroform-methanol, 24:1, v/v having Rf value 0.45. Bioassay of HPLC fractions confirms the presence of antibiotics picks at retention time: 3.24 minutes. The UV-Visible and mass spectra of the compound revealed that the active compound was different from other known antibiotics. The lowest minimum inhibitory concentration was recorded against (30 µg/mL). : The result suggests that a broad-spectrum antibacterial compound obtained from halophilic actinomycetes is effective against pathogenic bacteria. This compound may be a good alternative treatment against antibiotic-resistant pathogen .
抗生素耐药细菌的必然出现是一个全球健康问题。这些病原体削弱了现有抗生素的效用，是社区获得性感染的主要原因之一。本研究的目的是评估海洋放线菌产生抗病原体抗菌剂的能力。方法：分离、鉴定嗜盐放线菌，并筛选其对病原菌产生抗菌剂的能力。通过溶剂萃取法提取抗菌化合物，并通过基于薄层色谱的生物自显影法进行分离。抗菌化合物通过快速色谱法进一步纯化，随后采用高效液相色谱（HPLC）技术。通过光谱技术对活性组分进行表征。测定抗生素对病原体的最低抑菌浓度。结果：从海水中分离出一株新的嗜盐放线菌菌株rsk4。根据其生理、生化特征和基于16S rDNA序列的特征，将其命名为[具体名称]。它能够产生广谱抗菌化合物，并对耐药菌表现出显著的抑制活性。抗菌化合物在淀粉酪蛋白培养基中，5%NaCl和中性pH条件下，稳定期时分泌最佳。粗乙酸乙酯提取物用氯仿-甲醇（24:1，v/v）分离，Rf值为0.45。HPLC馏分的生物测定证实了在保留时间3.24分钟处存在抗生素峰。该化合物的紫外可见光谱和质谱表明，活性化合物与其他已知抗生素不同。对[具体病原菌]的最低抑菌浓度最低（30μg/mL）。结论：结果表明，从嗜盐放线菌中获得的广谱抗菌化合物对病原菌有效。该化合物可能是对抗耐药病原体的一种良好替代治疗方法。
