Alhambra Crisline Mae, Dhital Sushil, Sreenivasulu Nese, Butardo Vito M
International Rice Research Institute, Los Baños, Laguna, Philippines.
ARC Centre of Excellence in Plant Cell Walls, Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD, Australia.
Methods Mol Biol. 2019;1892:241-252. doi: 10.1007/978-1-4939-8914-0_13.
Rice is one of the staple foods which serves as the major source of carbohydrate in the human diet. A typical milled rice grain is mainly composed of starch of up to 80-90%, with an average of 6-8% proteins and some trace amounts of dietary fiber. Although cooked white rice can elicit variable glycemic response, a portion of rice starch may evade digestion in the human small intestine. The digested portion of rice can be estimated and characterized in vitro based on starch digestion extent and rate (kinetics). The indigestible portion of starch can also be quantified. This chapter will present micro-scale methods to quantify rice starch digestion rate and extent based on the sugar fractions released after treating the samples with digestive enzymes.
大米是人类饮食中碳水化合物的主要来源之一,是主食之一。典型的碾磨大米颗粒主要由高达80-90%的淀粉组成,平均含有6-8%的蛋白质和少量膳食纤维。尽管煮熟的白米会引发不同的血糖反应,但一部分大米淀粉可能会在人类小肠中逃避消化。基于淀粉消化程度和速率(动力学),可以在体外估计和表征大米的消化部分。淀粉的不可消化部分也可以进行量化。本章将介绍基于用消化酶处理样品后释放的糖组分来量化大米淀粉消化速率和程度的微观方法。