Department of Neurosurgery, Lariboisière Hospital, Paris, France.
Paris 7 Diderot University, Paris, France.
J Neurovirol. 2019 Feb;25(1):50-56. doi: 10.1007/s13365-018-0683-8. Epub 2018 Nov 5.
The role of the human cytomegalovirus (HCMV) in gliomagenesis is largely debated. Contradictory data exist regarding the sensitivity and specificity of HCMV detection techniques, including immunohistochemistry (IHC), in situ hybridization (ISH), and RNA and DNA sequencing. The aim of this study is to detect HCMV in glioblastoma (GBM) tumor samples using IHC, ISH, and real-time PCR (qPCR), as well as to correlate the findings with serological status and HCMV DNA load in blood. Forty-seven patients with histopathological diagnosis of GBM and HCMV serological status were retrospectively reviewed. HCMV DNA quantification in whole blood was performed in 31 patients. The detection of HCMV in tumor samples was performed using IHC in 42 cases, ISH in 10 cases, and qPCR in 29 cases. All but two patients were taking high steroid doses at the time of biological testing. HCMV seroprevalence was 68%. Active infection with HCMV DNA detected in blood was diagnosed in 6 out of 21 (28%) seropositive patients. HCMV was not detected in GBM samples using IHC or ISH, while qPCR was positive in one case (also positive for blood HCMV DNA). These data do not support a crucial role of HCMV in GBM tumorigenesis. HCMV might be reactivated in GBM patients, due to steroid treatment.
人巨细胞病毒（HCMV）在胶质瘤发生中的作用仍存在争议。在检测技术方面，包括免疫组织化学（IHC）、原位杂交（ISH）和 RNA 和 DNA 测序，存在相互矛盾的数据，这些技术检测 HCMV 的敏感性和特异性各不相同。本研究旨在使用 IHC、ISH 和实时 PCR（qPCR）检测胶质母细胞瘤（GBM）肿瘤样本中的 HCMV，并将检测结果与血清学状态和血液中的 HCMV DNA 载量相关联。回顾性分析了 47 例具有 GBM 组织病理学诊断和 HCMV 血清学状态的患者。对 31 例患者进行了全血 HCMV DNA 定量检测。在 42 例肿瘤样本中进行了 IHC 检测、10 例 ISH 检测和 29 例 qPCR 检测。除了两名患者外，所有患者在进行生物检测时均服用高剂量类固醇。HCMV 血清阳性率为 68%。在 21 例血清阳性患者中，有 6 例（28%）被诊断为血液 HCMV DNA 活动性感染。使用 IHC 或 ISH 未在 GBM 样本中检测到 HCMV，而 qPCR 在 1 例中呈阳性（血液 HCMV DNA 也呈阳性）。这些数据不支持 HCMV 在 GBM 肿瘤发生中起关键作用。由于类固醇治疗，HCMV 可能在 GBM 患者中重新激活。
