一种涉及PARP3和碱基切除修复蛋白的新型DNA断裂修复途径。

A New DNA Break Repair Pathway Involving PARP3 and Base Excision Repair Proteins.

作者信息

Belousova E A, Kutuzov M M, Ivankina P A, Ishchenko A A, Lavrik O I

机构信息

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia.

Novosibirsk State University, Novosibirsk, 630090, Russia.

出版信息

Dokl Biochem Biophys. 2018 Sep;482(1):233-237. doi: 10.1134/S1607672918050010. Epub 2018 Nov 5.

DOI:10.1134/S1607672918050010

PMID:30397881

Abstract

Poly(ADP-ribosyl)ation, which is catalyzed by PARP family proteins, is one of the main reactions in the cell response to genomic DNA damage. Massive impact of DNA-damaging agents (such as oxidative stress and ionizing radiation) causes numerous breaks in DNA. In this case, the development of a fast cell response, which allows the genomic DNA integrity to be retained, may be more important than the repair by more accurate but long-term restoration of the DNA structure. This is the first study to show the possibility of eliminating DNA breaks through their PARP3-dependent mono(ADP-ribosyl)ation followed by ligation and repair of the formed ribo-AP sites by the base excision repair (BER) enzyme complex. Taken together, the results of the studies on ADP-ribosylation of DNA and the data obtained in this study suggest that PARP3 may be a component of the DNA break repair system involving the BER enzyme complex.

摘要

由聚（ADP - 核糖）聚合酶（PARP）家族蛋白催化的聚（ADP - 核糖）基化作用是细胞对基因组DNA损伤作出反应的主要反应之一。DNA损伤剂（如氧化应激和电离辐射）的巨大影响会导致DNA出现大量断裂。在这种情况下，快速的细胞反应的发生可能比通过更精确但长期的DNA结构修复来修复更为重要，这种快速细胞反应能够保持基因组DNA的完整性。这是第一项表明通过PARP3依赖性单（ADP - 核糖）基化作用消除DNA断裂，随后由碱基切除修复（BER）酶复合物连接并修复形成的核糖 - AP位点的可能性的研究。综上所述，关于DNA的ADP - 核糖基化的研究结果以及本研究中获得的数据表明，PARP3可能是涉及BER酶复合物的DNA断裂修复系统的一个组成部分。