Plante G E, Prévost C, Chainey A, Braquet P, Sirois P
Am J Physiol. 1987 Aug;253(2 Pt 2):R375-8. doi: 10.1152/ajpregu.1987.253.2.R375.
The effect of increasing doses of prestegane B, a synthetic lignan, was examined in the anesthetized normal rat, using clearance methodology. Increasing doses of prestegane B 0.5, 1.0, 2.0, and 5.0 mg) were administered intravenously in our separate groups of hydropenic rats. Urine flow increased by 2.8 +/- 0.3, 4.5 +/- 0.5, 7.7 +/- 0.5, and 18.2 +/- 0.8 microliters/min, respectively, above control values. The rise of urinary sodium secretion was of similar magnitude and averaged 0.4 +/- 0.1, 0.8 +/- 0.2, 1.1 +/- 0.3, and 2.4 +/- 0.3 mu eq/min, respectively. No significant change in urinary phosphate excretion was obtained in all groups of rats, and glomerular filtration rate remained constant from control to experimental clearance periods. The natriuretic effect of prestegane B observed in this in vivo model could be related to the inhibition of the Na+-K+-adenosine triphosphate activity demonstrated in vitro in previous studies from our laboratory. The action of this substance is likely to be situated beyond the proximal tubule, since urinary phosphate was not altered. Prestegane B mimics the effects of other endogenous diuretic and natriuretic hormones, but its site of action and its effect on renal hemodynamics are obviously different.
采用清除率方法，在麻醉的正常大鼠中研究了合成木脂素前胡丙素剂量增加的效果。在单独的几组禁水大鼠中静脉注射递增剂量的前胡丙素（0.5、1.0、2.0和5.0毫克）。尿流量分别比对照值增加了2.8±0.3、4.5±0.5、7.7±0.5和18.2±0.8微升/分钟。尿钠分泌的增加幅度相似，平均分别为0.4±0.1、0.8±0.2、1.1±0.3和2.4±0.3微当量/分钟。所有大鼠组的尿磷排泄均无显著变化，从对照期到实验清除期，肾小球滤过率保持恒定。在这个体内模型中观察到的前胡丙素的利钠作用可能与我们实验室先前体外研究中证明的钠钾三磷酸腺苷活性的抑制有关。由于尿磷没有改变，这种物质的作用可能位于近端小管之外。前胡丙素模拟了其他内源性利尿和利钠激素的作用，但其作用部位及其对肾血流动力学的影响明显不同。
