Department of Pediatrics, Jagiellonian University Medical College, Krakow, Poland.
Chair of Pharmacology, Jagiellonian University Medical College, Krakow, Poland.
Invest Ophthalmol Vis Sci. 2018 Nov 1;59(13):5312-5319. doi: 10.1167/iovs.18-24914.
Retinopathy of prematurity (ROP) is a vision-threatening complication of a premature birth, in which the etiology still remains unclear. Importantly, the molecular processes that govern these effects can be investigated in a perturbed plasma proteome composition. Thus, plasma proteomics may add new insights into a better understanding of the pathogenesis of this disease.
The cord and peripheral blood of neonates (≤30 weeks gestational age) was drawn at birth and at the 36th postmenstrual week (PMA), respectively. Blood samples were retrospectively subdivided into ROP(+) and ROP(-) groups, according to the development of ROP.
The quantitative analysis of plasma proteome at both time points revealed 30 protein abundance changes between ROP(+) and ROP(-) groups. After standardization to gestational age, children who developed ROP were characterized by an increased C3 complement component and fibrinogen level at both analyzed time points.
Higher levels of the complement C3 component and fibrinogen, present in the cord blood and persistent to 36 PMA, may indicate a chronic low-grade systemic inflammation and hypercoagulable state that may play a role in the development of ROP.
早产儿视网膜病变（ROP）是一种早产儿出生时视力受损的并发症，其病因仍不清楚。重要的是，可在受干扰的血浆蛋白质组组成中研究控制这些影响的分子过程。因此，血浆蛋白质组学可能为更好地了解该疾病的发病机制提供新的见解。
分别在出生时和第 36 个孕周（PMA）时采集新生儿（<30 孕周）的脐带和外周血。根据 ROP 的发展，回顾性地将血液样本分为 ROP（+）和 ROP（-）组。
两个时间点的血浆蛋白质组的定量分析显示，ROP（+）和 ROP（-）组之间有 30 种蛋白质丰度发生变化。标准化至胎龄后，在两个分析时间点，患有 ROP 的儿童的补体 C3 成分和纤维蛋白原水平均升高。
脐带血中存在较高水平的补体 C3 成分和纤维蛋白原，并持续到 36 PMA，这可能表明存在慢性低度全身性炎症和高凝状态，这可能在 ROP 的发展中起作用。
