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3'-O-β-D-核糖呋喃基腺嘌呤在改变植物免疫中的作用。

A role for 3'-O-β-D-ribofuranosyladenosine in altering plant immunity.

机构信息

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Str. 32, Moscow, 119991, Russian Federation.

Imperial College London, Exhibition Road, London, SW7 2AZ, United Kingdom.

出版信息

Phytochemistry. 2019 Jan;157:128-134. doi: 10.1016/j.phytochem.2018.10.016. Epub 2018 Nov 3.

DOI:10.1016/j.phytochem.2018.10.016
PMID:30399495
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6290457/
Abstract

Our understanding of how, and the extent to which, phytopathogens reconfigure host metabolic pathways to enhance virulence is remarkably limited. Here we investigate the dynamics of the natural disaccharide nucleoside, 3'-O-β-D-ribofuranosyladenosine, in leaves of Arabidopsis thaliana infected with virulent Pseudomonas syringae pv. tomato strain DC3000. 3'-O-β-D-ribofuranosyladenosine is a plant derived molecule that rapidly accumulates following delivery of P. syringae type III effectors to represent a major component of the infected leaf metabolome. We report the first synthesis of 3'-O-β-D-ribofuranosyladenosine using a method involving the condensation of a small excess of 1-O-acetyl-2,3,5-three-O-benzoyl-β-ribofuranose activated with tin tetrachloride with 2',5'-di-O-tert-butyldimethylsilyladenosine in 1,2-dichloroethane with further removal of silyl and benzoyl protecting groups. Interestingly, application of synthetic 3'-O-β-D-ribofuranosyladenosine did not affect either bacterial multiplication or infection dynamics suggesting a major reconfiguration of metabolism during pathogenesis and a heavy metabolic burden on the infected plant.

摘要

我们对于植物病原菌如何以及在何种程度上重新配置宿主代谢途径以增强毒力的理解非常有限。在这里,我们研究了在感染了强毒力的丁香假单胞菌 pv.番茄菌株 DC3000 的拟南芥叶片中,天然二糖核苷 3'-O-β-D-核糖腺苷的动态变化。3'-O-β-D-核糖腺苷是一种植物衍生的分子,在向拟南芥叶片输送 P. syringae Ⅲ型效应物后迅速积累,代表了受感染叶片代谢组的主要成分。我们首次使用一种方法合成了 3'-O-β-D-核糖腺苷,该方法涉及用氯化亚锡活化的 1-O-乙酰基-2,3,5-三-O-苯甲酰基-β-核糖呋喃糖与 2',5'-二-O-叔丁基二甲基甲硅烷基腺苷在 1,2-二氯乙烷中进行缩合,然后去除硅烷基和苯甲酰基保护基团。有趣的是,合成的 3'-O-β-D-核糖腺苷的应用并没有影响细菌的增殖或感染动力学,这表明在发病过程中代谢发生了重大重排,感染植物的代谢负担很重。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d818/6290457/97c47cbb3ff1/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d818/6290457/05cccf1076a7/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d818/6290457/78776416216d/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d818/6290457/9d7e08de09b1/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d818/6290457/97c47cbb3ff1/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d818/6290457/05cccf1076a7/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d818/6290457/78776416216d/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d818/6290457/9d7e08de09b1/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d818/6290457/97c47cbb3ff1/gr4.jpg

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Function, Discovery, and Exploitation of Plant Pattern Recognition Receptors for Broad-Spectrum Disease Resistance.植物模式识别受体的功能、发现和广谱抗病性的利用。
Annu Rev Phytopathol. 2017 Aug 4;55:257-286. doi: 10.1146/annurev-phyto-080614-120106. Epub 2017 Jun 15.
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NAD Acts as an Integral Regulator of Multiple Defense Layers.
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