Zhu Zhongming, Li Feng, Zhong Fei, Zhai Kang, Tao Wei, Sun Gengyun
Pulmonary Department, First Affiliated Hospital of Anhui Medical University, Hefei 230022, China.
Department of respiration, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China.
Micromachines (Basel). 2017 Sep 27;8(10):291. doi: 10.3390/mi8100291.
Calcium phosphate nanoparticles (CPNPs) encapsulating small organic molecules, such as imaging agents and drugs, are considered to be ideal devices for cancer diagnosis or therapy. However, it is generally difficult to encapsulate small organic molecules in CPNPs because of the lack of solubility in water or binding affinity to calcium phosphate. To solve these issues, we utilized the carboxymethyl β-cyclodextrin (CM-β-CD) to increase the solubility and binding affinity to small organic molecules for the encapsulation into CPNPs in this work. The results indicated that the model molecules, hydrophilic rhodamine B (RB) and hydrophobic docetaxel (Dtxl), are successfully encapsulated into CPNPs with the assistance of CM-β-CD. We also demonstrated the CPNPs could be remarkably internalized into A549 cells, resulting in the efficient inhibition of tumor cells' growth.
包裹着诸如成像剂和药物等小分子的磷酸钙纳米颗粒（CPNP）被认为是癌症诊断或治疗的理想载体。然而，由于小分子在水中缺乏溶解性或与磷酸钙的结合亲和力，通常很难将其包裹在CPNP中。为了解决这些问题，我们在这项工作中利用羧甲基β-环糊精（CM-β-CD）来提高小分子的溶解性和结合亲和力，以便将其包裹进CPNP。结果表明，在CM-β-CD的辅助下，模型分子亲水性的罗丹明B（RB）和疏水性的多西他赛（Dtxl）成功地被包裹进CPNP。我们还证明了CPNP能够显著地被内化进A549细胞，从而有效抑制肿瘤细胞的生长。
