AluI、Cfr6I和PvuII限制修饰酶与含有N4-甲基胞嘧啶或5-甲基胞嘧啶的底物之间的相互作用。
Interaction of AluI, Cfr6I and PvuII restriction-modification enzymes with substrates containing either N4-methylcytosine or 5-methylcytosine.
作者信息
Butkus V, Klimasauskas S, Petrauskiene L, Maneliene Z, Lebionka A, Janulaitis A
出版信息
Biochim Biophys Acta. 1987 Aug 25;909(3):201-7. doi: 10.1016/0167-4781(87)90078-9.
The cleavage specificity of R.Cfr6I, an isoschizomer of PvuII restriction endonuclease was determined to be 5'CAG decreases CTG and the methylation specificity of Cfr6I and PvuII methylases, 5'CAG4mCTG. Thus, M.Cfr6I and M.PvuII are new additions to the list of methylases with N4-methylcytosine specificity. Neither of the above RM enzymes acts on the substrates containing either N4-methylcytosine or 5-methylcytosine in a cognate methylation position.
已确定PvuII限制性内切核酸酶的同裂酶R.Cfr6I的切割特异性为5'CAG↓CTG，以及Cfr6I和PvuII甲基化酶的甲基化特异性为5'CAG4mCTG。因此，M.Cfr6I和M.PvuII是具有N4-甲基胞嘧啶特异性的甲基化酶列表中的新成员。上述两种RM酶均不会作用于同源甲基化位置含有N4-甲基胞嘧啶或5-甲基胞嘧啶的底物。
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