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含N6-甲基腺嘌呤、N4-甲基胞嘧啶和5-甲基胞嘧啶的脱氧核糖核苷酸的合成:限制性内切酶的识别与切割(核苷与核苷酸第74部分)

Synthesis of decadeoxyribonucleotides containing N6-methyladenine, N4-methylcytosine, and 5-methylcytosine: recognition and cleavage by restriction endonucleases (nucleosides and nucleotides part 74).

作者信息

Ono A, Ueda T

出版信息

Nucleic Acids Res. 1987 Jan 12;15(1):219-32. doi: 10.1093/nar/15.1.219.

Abstract

The naturally-occurring modified bases, N6-methyladenine, N4-methylcytosine, and 5-methylcytosine were chemically introduced in place of the adenine or cytosine in the decadeoxyribonucleotides containing recognition sequences of Bgl II, Sau 3AI, Mbo I and Mfl I. The modified oligomers bind to the enzymes but the rates of cleavage by the enzymes are variable.

摘要

将天然存在的修饰碱基N6-甲基腺嘌呤、N4-甲基胞嘧啶和5-甲基胞嘧啶化学引入到含有Bgl II、Sau 3AI、Mbo I和Mfl I识别序列的脱氧核糖核苷酸中,取代腺嘌呤或胞嘧啶。修饰后的寡聚物与这些酶结合,但酶的切割速率各不相同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c85/340406/aff9cfddef61/nar00245-0234-a.jpg

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