Ono A, Ueda T
Nucleic Acids Res. 1987 Jan 12;15(1):219-32. doi: 10.1093/nar/15.1.219.
The naturally-occurring modified bases, N6-methyladenine, N4-methylcytosine, and 5-methylcytosine were chemically introduced in place of the adenine or cytosine in the decadeoxyribonucleotides containing recognition sequences of Bgl II, Sau 3AI, Mbo I and Mfl I. The modified oligomers bind to the enzymes but the rates of cleavage by the enzymes are variable.
将天然存在的修饰碱基N6-甲基腺嘌呤、N4-甲基胞嘧啶和5-甲基胞嘧啶化学引入到含有Bgl II、Sau 3AI、Mbo I和Mfl I识别序列的脱氧核糖核苷酸中,取代腺嘌呤或胞嘧啶。修饰后的寡聚物与这些酶结合,但酶的切割速率各不相同。