Myint P, Deeble D J, Beaumont P C, Blake S M, Phillips G O
Biochim Biophys Acta. 1987 Aug 13;925(2):194-202. doi: 10.1016/0304-4165(87)90109-7.
The reactions of the primary water radicals with the biopolymer hyaluronic acid have been studied by pulse radiolysis. Bimolecular rate constants, expressed in terms of the disaccharide repeating sub-unit of hyaluronic acid, for OH., H. and eaq- were found to be 7 X 10(8) M-1 X s-1, 5 X 10(7) M-1 X s-1 and less than 5 X 10(6) M-1 X s-1, respectively. By comparing the viscosities of samples, gamma-irradiated in the steady state under a variety of conditions, with unirradiated controls, the efficiencies with which selected radicals cause chain breakage have been determined. Efficiencies of 30%, 15%, 0%, 0.2% and 5% were estimated for OH., H., eaq-, methanol radicals and tert-butanol radicals, respectively. The presence of oxygen during irradiation increased the extent of chain breakage by a factor of 1.75.
通过脉冲辐解研究了初级水自由基与生物聚合物透明质酸的反应。以透明质酸的二糖重复亚基表示，OH·、H·和水合电子的双分子速率常数分别为7×10⁸ M⁻¹·s⁻¹、5×10⁷ M⁻¹·s⁻¹和小于5×10⁶ M⁻¹·s⁻¹。通过比较在各种条件下稳态γ辐照的样品与未辐照对照的粘度，确定了选定自由基引起链断裂的效率。OH·、H·、水合电子、甲醇自由基和叔丁醇自由基的效率分别估计为30%、15%、0%、0.2%和5%。辐照过程中氧气的存在使链断裂程度增加了1.75倍。
