Department of Orthodontics, School of Stomatology, Shandong University, Jinan, 250012, Shandong, China.
Department of the Oral and Maxillofacial Surgery, The Children's Hospital of Kunming, Kunming, 650500, Yunnan, China.
Biochem Biophys Res Commun. 2018 Dec 2;506(4):950-955. doi: 10.1016/j.bbrc.2018.10.163. Epub 2018 Nov 3.
To explore Girdin/Akt pathway protein expression and morphology change by cyclic tension in the periodontal ligament cells. Human periodontal ligament cells were exposed to cyclic tension force at 4000 μstrain and 0.5 Hz for 6 h though a four-point bending system. Cyclic tension force upregulated F-actin, Girdin and Akt expression in hPDL. In transmission electron microscope assay showed that there are more and bigger mitochondria, more and longer cynapses, more cellular organisms after tension force stimulation than control. The actin filament was changed to be regular lines and pointed to poles of cells. However, we found that the Girdin-depleted cells are small and there are more micro-organisms including more lysosomes and matrix vesicles than control. These finding suggest that the STAT3/Girdin/Akt pathway in PDL to response to mechanical stimulation as well, and Girdin may play a significant role in triggering cell proliferation and migration during orthodontic treatment. It provided an insight into the molecular basis for development of a vitro cell model in studying orthodontic treatment.
探讨牙周膜细胞在周期性张力作用下 Girdin/Akt 通路蛋白表达和形态变化。通过四点弯曲系统，将人牙周膜细胞暴露于 4000μstrain 和 0.5Hz 的周期性张力下 6 小时。周期性张力增加了 hPDL 中的 F-肌动蛋白、Girdin 和 Akt 的表达。在透射电镜检测中发现，张力刺激后细胞内有更多更大的线粒体、更多更长的突触、更多的细胞体。肌动蛋白纤维变为规则的线条并指向细胞的两极。然而，我们发现 Girdin 耗竭细胞较小，细胞内有更多的微生物，包括更多的溶酶体和基质小泡，比对照组多。这些发现表明，牙周膜中的 STAT3/Girdin/Akt 通路也能对机械刺激做出反应，Girdin 在触发正畸治疗期间的细胞增殖和迁移中可能发挥重要作用。这为研究正畸治疗的体外细胞模型的发展提供了分子基础。
