Barik S, Ghosh B, Whalen W, Lazinski D, Das A
Cell. 1987 Sep 11;50(6):885-99. doi: 10.1016/0092-8674(87)90515-0.
As a transcriptional activator, the N protein of phage lambda acts to suppress transcription termination by recognizing a promoter-proximal site, nut, which is separated from the terminators by thousands of base pairs. We demonstrate here that N interacts with the elongating RNA polymerase in transit through the boxB domain of nut. This interaction leads to the stable association of N as an integral component of the transcription apparatus. During subsequent elongation, N translocates along with polymerase through several defined terminators positioned beyond nut. Therefore, by being an operon-specific subunit of the transcription apparatus, N presumably prevents the interaction of polymerase with termination signals.
作为一种转录激活因子，噬菌体λ的N蛋白通过识别一个启动子近端位点（nut）来抑制转录终止，该位点与终止子相隔数千个碱基对。我们在此证明，N在通过nut的boxB结构域时与正在延伸的RNA聚合酶相互作用。这种相互作用导致N作为转录装置的一个组成部分稳定结合。在随后的延伸过程中，N与聚合酶一起穿过位于nut下游的几个特定终止子。因此，作为转录装置的一个操纵子特异性亚基，N大概阻止了聚合酶与终止信号的相互作用。
