Department of Chemistry, School of Chemistry and Materials Science, University of Science and Technology of China (USTC), Hefei, Anhui, 230026, People's Republic of China.
National Institute of Metrology, Beijing, 100013, People's Republic of China.
J Am Soc Mass Spectrom. 2019 Feb;30(2):368-375. doi: 10.1007/s13361-018-2082-z. Epub 2018 Nov 6.
A modified version of desorption electrospray ionization mass spectrometry was developed for (i) better utilization of analyte ions and (ii) larger sampling area via synchronization the pulsed nebulizer gas with ion injection. To synchronize the sheath gas, gas flow was paused for 50 ms within each cycle, leading to solvent accumulation at the end of emitter tip. That solvent accumulation enlarged the desorption areas. As a result, the amount of analytes increased. Thus, the improved signal intensity (~ 2-5-folds for various substrates) was benefit from both better analyte ion utilization and larger desorption areas. Finally, the enhanced signal intensity was confirmed with both garlic homogenate and brain homogenate. Graphical Abstract ᅟ.
开发了一种改进的解吸电喷雾电离质谱法，通过将脉冲雾化器气体与离子注入同步，实现了（i）更好地利用分析物离子和（ii）更大的采样面积。为了同步鞘气，在每个循环内将气流暂停 50 ms，导致在喷针尖的末端处积累溶剂。这种溶剂积累扩大了解吸区域。结果，分析物的量增加。因此，改进的信号强度（对于各种基质约为 2-5 倍）受益于更好的分析物离子利用和更大的解吸区域。最后，用大蒜匀浆和脑匀浆证实了增强的信号强度。
