Department of Colorectal Surgery, Department of Internal Medicine, Department of Gastric Surgery; Cancer Hospital of China Medical University, Liaoning Cancer Hospital and Insititute, Shenyang, Liaoning Province, P.R. China.
Eur Rev Med Pharmacol Sci. 2018 Oct;22(20):6801-6808. doi: 10.26355/eurrev_201810_16146.
Long non-coding RNAs (lncRNAs) have recently been identified as crucial regulators in colorectal cancer (CRC) progression. The aim of the study is to investigate the clinical role and biological effects of long non-coding RNA EWSAT1 in CRC.
The expression of lncRNA EWSAT1 was detected in 106 cases of fresh CRC tissues and matched adjacent normal tissues by qRT-PCR analyses. The Kaplan-Meier analysis and log-rank test were used to assess the association between lncRNA EWSAT1 expression and overall survival (OS) rate of CRC patients. Cell proliferation and invasion capacity were evaluated by CCK8 assay, colony formation, and transwell invasion assays. The protein expression was detected using western blot analysis.
LncRNA EWSAT1 expression was abnormally higher in CRC tissues compared to matched adjacent normal tissues. Higher lncRNA EWSAT1 expression significantly associated with depth of invasion, lymph node metastasis, and advanced tumor-node-metastasis (TNM) stage in CRC patients. Patients with higher EWSAT1 expression exhibited shorter OS compared with patients with lower EWSAT1 expression. Furthermore, lncRNA EWSAT1 knockdown significantly suppressed cell proliferation and invasion in CRC. In addition, lncRNA EWSAT1 knockdown suppressed cell epithelial-mesenchymal transition (EMT) through reducing Snail1, Snail2, and N-cadherin expression, but increasing E-cadherin expression in CRC cells.
Downregulation of lncRNA EWSAT1 suppressed cell proliferation and invasion of CRC, which indicated that EWSAT1 may be a potential target of CRC treatment.
长链非编码 RNA（lncRNA）最近被鉴定为结直肠癌（CRC）进展的关键调节因子。本研究旨在探讨 lncRNA EWSAT1 在 CRC 中的临床作用和生物学效应。
通过 qRT-PCR 分析检测 106 例新鲜 CRC 组织和配对的相邻正常组织中 lncRNA EWSAT1 的表达。采用 Kaplan-Meier 分析和对数秩检验评估 lncRNA EWSAT1 表达与 CRC 患者总生存率（OS）率的相关性。通过 CCK8 assay、集落形成和 Transwell 侵袭实验评估细胞增殖和侵袭能力。使用 Western blot 分析检测蛋白表达。
与配对的相邻正常组织相比，CRC 组织中 lncRNA EWSAT1 的表达异常升高。较高的 lncRNA EWSAT1 表达与 CRC 患者的浸润深度、淋巴结转移和晚期肿瘤-淋巴结-转移（TNM）分期显著相关。与 EWSAT1 表达较低的患者相比，表达较高 EWSAT1 的患者 OS 较短。此外，lncRNA EWSAT1 下调显著抑制 CRC 细胞的增殖和侵袭。此外，lncRNA EWSAT1 下调通过降低 Snail1、Snail2 和 N-钙粘蛋白表达，同时增加 CRC 细胞中 E-钙粘蛋白表达，抑制细胞上皮-间充质转化（EMT）。
下调 lncRNA EWSAT1 抑制 CRC 细胞的增殖和侵袭，表明 EWSAT1 可能是 CRC 治疗的潜在靶点。
