Han Li-Ping, Wang Cun-Ping, Han Si-Lin
Department of Orthopaedics, Zaozhuang Municipal Hospital, 41 Longtou Rd, Zaozhuang 277100, Shandong, P.R. China.
Med Sci (Paris). 2018 Oct;34 Focus issue F1:81-86. doi: 10.1051/medsci/201834f114. Epub 2018 Nov 7.
Osteosarcoma is the most common malignant bone tumor with high incidence in adolescence and poor prognosis. RBM10, a member of RBPs, was reported to be a tumor suppressor in many kinds of cancers. However, the roles of RBM10 in osteosarcoma remain unknown. In this study, we found that overexpression of RBM10 decreased osteosarcoma cell proliferation and colony formation in soft agar, and inhibited osteosarcoma cell migration and invasion. Our results also revealed that RBM10 overexpression induced osteosarcoma cell apoptosis via the inhibition of Bcl-2, the activation of caspase-3, and the transcription and production of TNF-α. Our results indicated that RBM10 acts as a tumor suppressor in osteosarcoma. This could enable to define a new strategy for diagnosis and treatment of patients with osteosarcoma.
骨肉瘤是最常见的恶性骨肿瘤，在青少年中发病率高且预后差。RBM10是RNA结合蛋白（RBPs）家族的成员之一，据报道在多种癌症中是一种肿瘤抑制因子。然而，RBM10在骨肉瘤中的作用仍不清楚。在本研究中，我们发现RBM10的过表达降低了骨肉瘤细胞的增殖以及在软琼脂中的集落形成，并抑制了骨肉瘤细胞的迁移和侵袭。我们的结果还显示，RBM10的过表达通过抑制Bcl-2、激活caspase-3以及TNF-α的转录和产生来诱导骨肉瘤细胞凋亡。我们的结果表明RBM10在骨肉瘤中作为一种肿瘤抑制因子发挥作用。这可能有助于为骨肉瘤患者确定一种新的诊断和治疗策略。
