Kanat Gurdal
Department of Environmental Engineering, Faculty of Civil Engineering, Yildiz Technical University, Istanbul, Turkey.
Cell Mol Biol (Noisy-le-grand). 2018 Oct 30;64(13):10-14.
The biodegradation of pyroxasulfone (C12H14F5N3O4S), which is the selective herbicide in wheat farming in Turkey is compared with some soil bacteria. These microorganisms were isolated in collected soil samples in Thrace region of Turkey from an agricultural area previously unexposed to pyroxasulfone. The microbial biodegradation of pyroxasulfone was investigated using liquid experiments with identified cultures to determine which of the microorganisms shows best removal performance under agitated culture conditions. The experiments continued about two weeks. Five different apparatuses, were set up and pyroxasulfone in 750 ppm concentration (advised concentration of wheat farmers) was added to each Erlenmayer flasks. Approximately 107CFU/ml of each bacteria added to these flasks. These flasks were shaken at 130rpm at 27 0C in sterile conditions for 8 days. Every day, each sample was collected by filtering from flasks and Chemical Oxygen Demand (COD), biochemical oxygen demand (BOD5), total organic carbon (TOC) was determined. As a result of the study, best removal performance observed in Bacillus thuringiensis and Fusarium fujikuroi as 91 and 93% at 7 days in COD, 88 and 83% in BOD5, 90 and 86% in TOC parameters. The lowest performance was seen on Clostridium tetani species for COD, BOD5 and TOC as 55%, 61% and 60% respectively on 7 days. The performance for Bacillus simplex and Bacillus megaterium species occurred between 70% and 80% for these three parameters.
在土耳其，用于小麦种植的选择性除草剂唑吡嘧磺隆（C12H14F5N3O4S）的生物降解与一些土壤细菌进行了比较。这些微生物是从土耳其色雷斯地区采集的土壤样本中分离出来的，该农业区域之前未接触过唑吡嘧磺隆。使用已鉴定培养物的液体实验研究了唑吡嘧磺隆的微生物降解，以确定哪种微生物在振荡培养条件下表现出最佳的去除性能。实验持续了约两周。设置了五个不同的装置，并向每个锥形瓶中加入浓度为750 ppm（小麦种植者建议浓度）的唑吡嘧磺隆。向这些锥形瓶中加入每种细菌约107CFU/ml。这些锥形瓶在无菌条件下于27℃以130rpm振荡8天。每天，通过过滤从锥形瓶中收集每个样品，并测定化学需氧量（COD）、生化需氧量（BOD5）和总有机碳（TOC）。研究结果表明，在第7天时，苏云金芽孢杆菌和藤仓镰刀菌的去除性能最佳，COD去除率分别为91%和93%，BOD5去除率分别为88%和83%，TOC去除率分别为90%和86%。破伤风梭菌在第7天时，COD、BOD5和TOC的去除性能最低，分别为55%、61%和60%。简单芽孢杆菌和巨大芽孢杆菌在这三个参数上的去除性能在70%至80%之间。
