Australian Herbicide Resistance Initiative, School of Agriculture and Environment, University of Western Australia, 35 Stirling Highway, Crawley 6009, Australia.
Separation Science and Mass Spectrometry Facility, University of Western Australia, 35 Stirling Highway, Crawley 6009, Australia.
J Agric Food Chem. 2024 Feb 28;72(8):3937-3948. doi: 10.1021/acs.jafc.3c08141. Epub 2024 Feb 14.
Resistance to the herbicide pyroxasulfone has slowly but steadily increased in agricultural weeds. The evolved resistance of one population has been attributed to the conjugation of pyroxasulfone to reduced glutathione, mediated by glutathione transferase (GST) activity. To determine if GST-based metabolism is a widespread mechanism of pyroxasulfone resistance in , a number of putative-resistant populations were screened for GST activity toward pyroxasulfone, the presence of GSTF13-like isoforms (previously implicated in pyroxasulfone conjugation in this species), tissue glutathione concentrations, and response to inhibitors of GSTs and oxygenases. Although there were no direct correlations between pyroxasulfone resistance levels and these individual parameters, a random forest analysis indicated that GST activity was of primary importance for resistance to this herbicide.
在农业杂草中，对除草剂吡唑磺隆的抗性已经缓慢但稳定地增加。一个种群的进化抗性归因于吡唑磺隆与还原型谷胱甘肽的共轭，由谷胱甘肽转移酶（GST）活性介导。为了确定 GST 代谢是否是杂草对吡唑磺隆产生抗性的一种普遍机制，对一些假定的抗性种群进行了 GST 对吡唑磺隆的活性、存在 GSTF13 样同工型（先前涉及该物种中吡唑磺隆共轭）、组织谷胱甘肽浓度以及对 GST 和加氧酶抑制剂的反应的筛选。尽管吡唑磺隆抗性水平与这些单个参数之间没有直接相关性，但随机森林分析表明，GST 活性对该除草剂的抗性至关重要。
