Taha Inass Mohamed, Abdu Allah Azza M, Abd El Gayed Eman Masoud
Department of Internal Medicine, Faculty of Medicine, Taibah University, KSA.
Medical Biochemistry and Molecular Biology Department, Faculty Of Medicine, Menoufia University, Egypt.
Cell Mol Biol (Noisy-le-grand). 2018 Oct 30;64(13):15-20.
Toll-like receptor 4 (TLR4) plays an important role in modulating innate immunity. Type 2 diabetes mellitus (T2DM) is a chronic disease that is characterized by impaired insulin resistance and abnormal immune response. Genetic background and consequently genetic factors might have a key role in both onset and progression of T2DM-related complications. The aim of this work was to study the role of toll-like receptor 4 (TLR4) in the development of type 2 diabetes mellitus (T2DM). This study was carried out on 90 subjects, 30 type 2 diabetic patients, 30 patients with impaired glucose tolerance and 30 age and gender matched healthy controls. mRNA expression of (TLR4) was assessed by reverse transcriptase PCR (RT-PCR) using real time PCR.. Results showed significant statistical difference between the three studied groups regarding BMI, serum FBG, HDL, TGs, TC, LDL, HOMA -IR and mRNA expression of TLR4 with highest level of TLR4 mRNA expression in T2DM patients. From this study, it might be concluded that high expression of (TLR4) is associated with T2DM.
Toll样受体4（TLR4）在调节固有免疫中起重要作用。2型糖尿病（T2DM）是一种以胰岛素抵抗受损和免疫反应异常为特征的慢性疾病。遗传背景以及由此产生的遗传因素可能在T2DM相关并发症的发生和发展中起关键作用。这项工作的目的是研究Toll样受体4（TLR4）在2型糖尿病（T2DM）发生发展中的作用。本研究对90名受试者进行，其中30名2型糖尿病患者，30名糖耐量受损患者以及30名年龄和性别匹配的健康对照者。使用实时PCR通过逆转录酶PCR（RT-PCR）评估（TLR4）的mRNA表达。结果显示，在BMI、血清空腹血糖（FBG）、高密度脂蛋白（HDL）、甘油三酯（TGs）、总胆固醇（TC）、低密度脂蛋白（LDL）、稳态模型评估胰岛素抵抗（HOMA-IR）以及TLR4的mRNA表达方面，三个研究组之间存在显著统计学差异，T2DM患者中TLR4 mRNA表达水平最高。从这项研究中可以得出结论，（TLR4）的高表达与T2DM相关。
