Biology Department, Faculty of Science, Taif University, Taif, Saudi Arabia.
Zoology Department, Faculty of Science, Zagazig University, Zagazig, Egypt.
PLoS One. 2018 Nov 7;13(11):e0204913. doi: 10.1371/journal.pone.0204913. eCollection 2018.
Aspartame (ASP) has been used as an alternative to sucrose for diabetics and obese people worldwide. Co-administration of L-carnitine (LC) with ASP has a protective effect against the liver and kidney toxicity induced of ASP. The goal of the investigation was to assess the enhancement of LC effect on the cardiac toxicity caused of ASP. The rats were divided into 6 groups: control with saline, LC (10 mg/kg), ASP (75 mg/kg), ASP (150 mg/kg), LC with 75 mg/kg of ASP, and LC with 150 mg/kg ASP. The antioxidants were determined by measuring the activities of myeloperoxidase, xanthine oxidase, superoxide dismutase, catalase, and glutathione peroxidase, and by assessing the levels of lipid peroxidation, total thiols, and glutathione. There was a significant elevation in LPO, in conjunction with a significant decline in the enzymatic antioxidants superoxide dismutase, catalase, and glutathione peroxidase and the non-enzymatic antioxidants glutathione and thiols. The cardiac myofibrils were found in a disarrayed pattern in ASP treated-animals as compared to the control rats. The animals treated with ASP-HD showed more than one apoptotic cell with a large tail and a small head, and the relaxed loops of the damaged DNA were extended to form a comet-shaped structure. These effects may be due to the excessive generation of reactive oxygen species by ASP, which reduces cardiac function. Co-administration of LC with ASP improved all of the above-mentioned parameters that were disrupted of ASP alone. This study evidences a sufficient originality in showing how LC plays a positive role against cardiac toxicity of ASP.
阿斯巴甜（ASP）已被用作全球糖尿病患者和肥胖人群的蔗糖替代品。左旋肉碱（LC）与 ASP 的联合使用具有对抗 ASP 诱导的肝和肾毒性的保护作用。本研究的目的是评估 LC 对 ASP 引起的心脏毒性的增强作用。将大鼠分为 6 组：生理盐水对照、LC（10mg/kg）、ASP（75mg/kg）、ASP（150mg/kg）、ASP 与 75mg/kg LC、ASP 与 150mg/kg LC。通过测量髓过氧化物酶、黄嘌呤氧化酶、超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性，以及评估脂质过氧化、总巯基和谷胱甘肽的水平来确定抗氧化剂。LPO 显著升高，同时超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶等酶抗氧化剂以及谷胱甘肽和巯基等非酶抗氧化剂显著下降。与对照组大鼠相比，ASP 处理的动物心肌纤维排列紊乱。与 ASP-HD 处理的动物表现出更多具有大尾巴和小头的凋亡细胞，并且受损 DNA 的松弛环延伸形成彗星状结构。这些影响可能是由于 ASP 产生的过量活性氧，从而降低了心脏功能。LC 与 ASP 联合使用改善了 ASP 单独使用破坏的所有上述参数。这项研究充分证明了 LC 如何发挥对抗 ASP 心脏毒性的积极作用。
