Pandey K N, Osteen K G, Inagami T
Endocrinology. 1987 Sep;121(3):1195-7. doi: 10.1210/endo-121-3-1195.
The effect of rat atrial natriuretic factor (ANFIV102-126) on the regulation of steroidogenesis in human G-L cells have been studied in vitro. The treatment of cells with ANF (1 X 10(-8)M) resulted in a 3- to 4-fold increase in progesterone secretion compared to the controls. ANF in combination with LH (200 ng/ml) increased progesterone secretion more than six-fold over controls. Concomitantly, ANF stimulated the accumulation of cGMP 30-fold, whereas the level of cAMP was either unchanged or fell slightly (10-15%). Mono[125I]-iodo-ANF bound to G-L cell surface specific receptors with a Kd of 1.8 X 10(-10)M at a density (Bmax) of 160,000-190,000 sites/cell. The binding of [125I]-iodo-ANF was competed by unlabeled ANF in a dose-dependent manner, and hormones unrelated to ANF, such as angiotensins, ACTH or LH, were unable to compete with [125I]-iodo-ANF. The results indicate that ANF can stimulate progesterone secretion and cGMP accumulation in ovarian G-L cells without affecting the level of cAMP and that the stimulatory effects of ANF on G-L cell steroidogenesis may proceed via mechanism(s) involving an intracellular messenger other than cAMP.
在体外研究了大鼠心房利钠因子（ANFIV102 - 126）对人G - L细胞类固醇生成调节的影响。与对照组相比，用ANF（1×10⁻⁸M）处理细胞导致孕酮分泌增加3至4倍。ANF与促黄体生成素（LH，200 ng/ml）联合使用使孕酮分泌比对照组增加超过6倍。同时，ANF刺激环磷酸鸟苷（cGMP）积累30倍，而环磷酸腺苷（cAMP）水平要么未改变，要么略有下降（10 - 15%）。单[¹²⁵I] - 碘 - ANF以1.8×10⁻¹⁰M的解离常数（Kd）和160,000 - 190,000个位点/细胞的密度（Bmax）与G - L细胞表面特异性受体结合。[¹²⁵I] - 碘 - ANF的结合被未标记的ANF以剂量依赖性方式竞争，而与ANF无关的激素，如血管紧张素、促肾上腺皮质激素（ACTH）或LH，不能与[¹²⁵I] - 碘 - ANF竞争。结果表明，ANF可刺激卵巢G - L细胞中的孕酮分泌和cGMP积累，而不影响cAMP水平，并且ANF对G - L细胞类固醇生成的刺激作用可能通过涉及cAMP以外的细胞内信使的机制进行。
