Department of Biology, University of Texas at San Antonio, San Antonio, TX 78249, USA; Genomics Core, University of Texas at San Antonio, San Antonio, TX 78249, USA.
Department of Biology, University of Texas at San Antonio, San Antonio, TX 78249, USA.
Cell Rep. 2018 Nov 6;25(6):1650-1667.e8. doi: 10.1016/j.celrep.2018.10.026.
Spermatogenesis is a complex and dynamic cellular differentiation process critical to male reproduction and sustained by spermatogonial stem cells (SSCs). Although patterns of gene expression have been described for aggregates of certain spermatogenic cell types, the full continuum of gene expression patterns underlying ongoing spermatogenesis in steady state was previously unclear. Here, we catalog single-cell transcriptomes for >62,000 individual spermatogenic cells from immature (postnatal day 6) and adult male mice and adult men. This allowed us to resolve SSC and progenitor spermatogonia, elucidate the full range of gene expression changes during male meiosis and spermiogenesis, and derive unique gene expression signatures for multiple mouse and human spermatogenic cell types and/or subtypes. These transcriptome datasets provide an information-rich resource for studies of SSCs, male meiosis, testicular cancer, male infertility, or contraceptive development, as well as a gene expression roadmap to be emulated in efforts to achieve spermatogenesis in vitro.
精子发生是一个复杂而动态的细胞分化过程，对男性生殖至关重要，并由精原干细胞（SSC）维持。虽然已经描述了某些精子细胞类型的聚集的基因表达模式，但以前尚不清楚持续精子发生的完整基因表达模式连续体。在这里，我们对来自未成熟（出生后第 6 天）和成年雄性小鼠和成年男性的超过 62,000 个单个精子发生细胞进行了单细胞转录组分析。这使我们能够解析 SSC 和祖细胞精原细胞，阐明雄性减数分裂和精子发生过程中的全部基因表达变化，并为多种小鼠和人类精子发生细胞类型和/或亚型得出独特的基因表达特征。这些转录组数据集为 SSC、雄性减数分裂、睾丸癌、男性不育或避孕开发的研究提供了丰富的信息资源，也是实现体外精子发生的基因表达路线图的效仿对象。
